Occurrence and nature of mixed infections in nodules of field-grown soybeans (Glycine max) |
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| Authors: | M Moawad E L Schmidt |
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| Institution: | (1) National Research Centre, Dokki, Cairo, Egypt;(2) Department of Soil Science, University of Minnesota, 55108 St. Paul, MN, USA |
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| Abstract: | Summary Mixed infections of Bradyrhizobium japonicum strains in early and late nodules of four soybean cultivars were studied in a field soil. Nodule occupants were identified by immunofluorescence using serogroup specific antibodies prepared against B. japonicum strains USDA 110, USDA 123, and USDA 138. Double infection was determined directly by combined examination of the same microscopic field by fluorescence and phase contrast microscopy. Double strain occupancy was observed consistently, and its occurrence did not differ substantially in pouch, soil pot, and field experiments, ranging in incidence from 12% to 32%. No significant differences in the incidence or nature of double infection could be attributed to cultivar, seed inoculation, or plant maturity. Strains reactive to strain USDA 123-fluorescent antibody were dominant in both singly and doubly infected nodules irrespective of cultivar, plant age, or seed inoculation with strain USDA 110.Paper no. 15092 in the Scientific Journal Series of the Minnesota Agricultural Experiment Station, St. Paul |
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| Keywords: | Mixed nodule infections Glycine max Soybeans Bradyrhizobium japonicum strains Field inoculation |
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