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不同激素对月季茎段扦插和组织培养的影响
引用本文:徐秋燕,杨平,张边江.不同激素对月季茎段扦插和组织培养的影响[J].湖北农业科学,2012,51(9):1904-1906.
作者姓名:徐秋燕  杨平  张边江
作者单位:南京晓庄学院生物化工与环境工程学院,南京,211171
基金项目:江苏省教育厅高校自然基金项目
摘    要:以月季(Rosa chinensis)茎段为实验材料,研究不同浓度的ABT、IBA对其扦插生根的影响以及6-BA、NAA、GA3对组织培养的影响,探讨适宜月季快速繁殖的条件.结果显示,MS培养基中添加50 mg/L的ABT或100 mg/L的IBA时,月季茎段生根率较高,生长健壮.MS+0.5 mg/L 6-BA+0.3 mg/L NAA是不定芽诱导的最适培养基;MS+1.0 mg/L GA3是不定芽生长的最适培养基.

关 键 词:月季(Rosa  chinensis)  组织培养  不定芽  增殖

Effect of Different Hormones on Rooting and Tissue Culture of Rosa chinensis Stem
XU Qiu-yan , YANG Ping , ZHANG Bian-jiang.Effect of Different Hormones on Rooting and Tissue Culture of Rosa chinensis Stem[J].Hubei Agricultural Sciences,2012,51(9):1904-1906.
Authors:XU Qiu-yan  YANG Ping  ZHANG Bian-jiang
Institution:(School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang College,Nanjing 211171,China)
Abstract:Using the stem of Rosa chinensis as experimental material,the effects of ABT and IBA concentration on its rooting,and 6-BA,NAA,GA3 concentration on the tissue culture were studied.The results showed that when 50 mg/L ABT or 100 mg/L IBA was added into MS,the rooting of stem cutting was relatively high,and the roots grew well.MS+0.5 mg/L 6-BA+0.3 mg/L NAA was the optimal medium for adventitious bud induction;MS+1.0mg/L GA3 was the optimal medium for adventitious bud growth.
Keywords:Rosa chinensis  tissue culture  bud  proliferation
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