| 庐山楼梯草总黄酮提取工艺的优化 |
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| 引用本文： | 杨建远,丁忠青,李志杰.庐山楼梯草总黄酮提取工艺的优化[J].湖北农业科学,2012,51(8):1663-1666,1669. |
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| 作者姓名： | 杨建远 丁忠青 李志杰 |
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| 作者单位： | 九江学院生命科学学院,江西九江,332000 |
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| 基金项目： | 九江学院2011年度大学生自主创新性实验项目 |
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| 摘 要： | 为研究庐山楼梯草(Elatostema stewardii Merr.)总黄酮的提取工艺,以乙醇溶液为溶剂,在单因素试验基础上利用响应曲面法的中心组合设计,对庐山楼梯草总黄酮提取工艺参数进行优化分析.结果表明,提取庐山楼梯草总黄酮的最优工艺条件为乙醇体积分数55％、提取温度80℃、液料比37∶1 (V/m,mL∶g)、提取时间2.4 h、提取2次,该条件下庐山楼梯草总黄酮提取率为5.14％.
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| 关 键 词： | 响应曲面法 庐山楼梯草(Elatostema stewardii Merr.) 总黄酮 提取工艺 |
Optimization of the Total Flavonoids Extraction Conditions from Elatostema stewardii |
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| YANG Jian-yuan
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DING Zhong-qing
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LI Zhi-jie.Optimization of the Total Flavonoids Extraction Conditions from Elatostema stewardii[J].Hubei Agricultural Sciences,2012,51(8):1663-1666,1669. |
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| Authors: | YANG Jian-yuan DING Zhong-qing LI Zhi-jie |
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| Institution: | (College of Life Sciences,Jiujiang University,Jiujiang 332000,Jiangxi,China) |
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| Abstract: | Total flavonoids were extracted from Elatostema stewardii Merr.using alcohol as solvent.Single factor tests as well as response surface methodology based on a central composite design was applied to optimize the extraction process.The results showed that the optimized extraction conditions were volume fraction of alcohol solvent,55%;extraction temperature,80 ℃;solvent to material ratio,37∶1(V/m,mL∶g);extraction time,2.4 h;and extract for twice.Under these extraction conditions,the extraction yield could be up to 5.14%. |
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| Keywords: | response surface methodology Elatostema stewardii Merr total flavonoids extraction condition |
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