| 5个家兔群体白细胞介素10基因外显子的遗传多样性分析 |
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| 引用本文： | 冒留留,潘雨来,王晓明,万小颖,李碧春,吴信生.5个家兔群体白细胞介素10基因外显子的遗传多样性分析[J].畜牧兽医学报,2012,43(5):723-728. |
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| 作者姓名： | 冒留留 潘雨来 王晓明 万小颖 李碧春 吴信生 |
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| 作者单位： | 1. 江苏省动物遗传繁育与分子设计重点实验室,扬州225009;扬州大学动物科学与技术学院,扬州225009
2. 江苏省金陵种兔场,南京,211103 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助，江苏省高新技术项目 |
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| 摘 要： | 本研究旨在分析家兔IL-10基因外显子的多态性,以期为进一步研究家兔IL-10与疾病抗性的相关性提供理论依据。根据GenBank上收录的IL-10基因序列设计5对特异性引物,采用PCR-SSCP方法对海狸色獭兔、白色獭兔、皖系长毛兔、闽西南黑兔、九疑山兔这5个家兔群体IL-10基因的5个外显子序列进行多态性分析。结果表明:在IL-10基因外显子3上检测到4种等位基因,10种基因型,存在3个SNPs位点;在外显子4上检测到2种等位基因,3种基因型,存在1个SNP位点。而外显子1、2、5对于实验群体未发现有遗传多态性。在外显子3中,D等位基因只在闽西南黑兔和九疑山兔中检测到,除海狸色獭兔和闽西南黑兔外其余群体均处于哈代-温伯格平衡,各群体不同基因型分布存在极显著差异(P<0.01)。在外显子4中,A1B1基因型在獭兔群体中没有检测到;除海狸色獭兔外其余各群体均处于哈代-温伯格平衡,海狸色獭兔跟白兔獭兔不同基因型分布差异不显著(P>0.05),而其余群体彼此不同基因型分布存在显著性差异(P<0.05或P<0.01)。结果提示:5个家兔群体在IL-10基因外显子3和4中存在遗传多态性,不同家兔群体在遗传基础上存在着一定的差异。
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| 关 键 词： | 家兔 IL-10基因 多态性 |
Genetic Diversity of IL-10 Gene Exons in 5 Rabbit Populations |
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| MAO Liu-liu
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PAN Yu-lai
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WANG Xiao-ming
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WAN Xiao-ying
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LI Bi-chun
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WU Xin-sheng.Genetic Diversity of IL-10 Gene Exons in 5 Rabbit Populations[J].Acta Veterinaria et Zootechnica Sinica,2012,43(5):723-728. |
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| Authors: | MAO Liu-liu PAN Yu-lai WANG Xiao-ming WAN Xiao-ying LI Bi-chun WU Xin-sheng |
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| Institution: | 1,2 (1.Jiangsu Key Laboratory of Animal Genetics,Breeding and Molecular Design,Yangzhou 225009, China;2.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009, China;3.Jiangsu Jinling Rabbits Field,Nanjing 211103,China) |
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| Abstract: | This experiment was conducted to study the polymorphism of IL-10 gene exons in rabbit and aimed to provide a theoretical foundation for further reseach on correlation between rabbit IL-10 gene and disease resistance.Five exons of IL-10 gene in 5 rabbit populations including Beaver Color Rex rabbit,White Rex rabbit,Wan-line Angora rabbit,Jiuyishan rabbit and Minxinan Black rabbit were scanned by PCR-SSCP method with five specific primers designed according to the IL-10 sequence from GenBank.The results showed that 10 genotypes and 4 alleles were found in exon3 of IL-10 gene which contained three SNPs,3 genotypes and 2 alleles were found in exon4 which contained one SNP.There was no mutation found at exon1,exon2,exon5 of IL-10 gene.In exon3,D allele was found only in Minxinan Black rabbit and Jiuyishan rabbit.The distribution of these genotypes in all populations was consistent with the Hardy-Weinberg equilibrium except Beaver Color Rex rabbit and Minxinan Black rabbit,which was very significantly different(P<0.01) among different populations.In exon4,A1B1 genotype was not found in Rex rabbit group.The distribution of these genotypes was significant different(P<0.05 or P<0.01) among different populations except between Beaver Color and White Rex rabbit(P>0.05).These results indicated that genetic diversity of rabbit IL-10 gene was detected in exon3 and exon4. |
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| Keywords: | rabbit IL-10 gene polymorphism |
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