| Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources |
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| 作者姓名： | Natalia Pimentel Esposito-Polesi Leandro Silva de Oliveira Francisco Jose Benedini Baccarin Cristina Vieira de Almeida Marcilio de Almeida |
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| 作者单位： | Biological Science Department;Institute of Agriculture Sciences;State University of Goias（UEG）;In vitro Palm Consultoria |
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| 基金项目： | The work was supported by the National Council of Technological and Scientific Development (CNPq) Process |
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| 摘 要： | Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous development.The responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation.
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| 关 键 词： | Plant tissue CULTURE EXPLANT SOURCES CULTURE media SUCROSE pH |
Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources |
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| Natalia Pimentel Esposito-Polesi,Leandro Silva de Oliveira,Francisco Jose Benedini Baccarin,Cristina Vieira de Almeida,Marcilio de Almeida.Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources[J].Journal of Forestry Research,2020,31(3):857-869. |
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| Institution: | Biological Science Department,″Luiz de Queiroz″ SuperiorCollege of Agriculture, University of S?o Paulo (ESALQ/USP), Pádua Dias Avenue, 11, P.O.Box 9, Piracicaba, SP 13418-900, Brazil;Institute of Agriculture Sciences, Federal University of Minas Gerais (UFMG), Universitária Avenue, 1000, Montes Claros, MG 39404-547, Brazil;State University of Goiás (UEG), Rua S7, Qd.1, S Sul, Palmeiras de Goiás, GO 76190-000, Brazil;In vitro Palm Consultoria, Estudo e Desenvolvimento Biológico Ltda, Rua Itajobi, 421, Piracicaba, SP 13432-000, Brazil |
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| Abstract: | Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous development.The responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation. |
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