| 根据线粒体保守序列检测鹿属动物源性成分 |
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| 引用本文： | 陈颖,吴亚君,徐宝梁,王晶,钱增敏,苏宁.根据线粒体保守序列检测鹿属动物源性成分[J].畜牧与兽医,2004,36(5):1-4. |
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| 作者姓名： | 陈颖 吴亚君 徐宝梁 王晶 钱增敏 苏宁 |
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| 作者单位： | 中国进出口商品检验技术研究所,北京,100025 |
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| 基金项目： | 科技部重要技术标准专项 (2 0 0 2BA90 6A2 5 0 8)，科技部社会公益研究专项 (2 0 2DIA50 0 34) |
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| 摘 要： | 本文根据鹿线粒体mtDNA细胞色素b中的保守序列 ,设计了针对鹿属动物的特异性扩增引物。通过聚合酶链反应 (PolymeraseChainReac tion,PCR)得到 1 94bp的特异片段 ,而其它动物如鸡、牛、羊、兔、猪、鱼、骆驼、马等中则无此条带。通过内切酶AlwI酶切可对扩增结果进行验证。该方法对鹿成分的检测低限为 0 1 % ,可作为饲料中鹿属动物源性成分鉴别检测的有效方法之一 ,也可作为鹿类药材真伪鉴别的方法。
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| 关 键 词： | 鹿属 线粒体 细胞色素b PCR |
| 文章编号： | 0529-5130(2004)05-0001-04 |
| 修稿时间： | 2003年8月22日 |
Determination of deer-derived materials with conserved mitochondrial cytochrome b sequence |
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| CHEN Ying,WU Ya-jun,XU Bao-liang,WANG Jing,QIAN Zeng-min,SU Ning.Determination of deer-derived materials with conserved mitochondrial cytochrome b sequence[J].Animal Husbandry & Veterinary Medicine,2004,36(5):1-4. |
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| Authors: | CHEN Ying WU Ya-jun XU Bao-liang WANG Jing QIAN Zeng-min SU Ning |
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| Abstract: | Species-specific PCR primer has been designed according to the sequence of Cytb gene sequence. The PCR amplified 194bp fragments from deer, but no fragments from chicken, bovine, sheep, goat, rabbit, pig, fish, camel and horse. The PCR result could be proved by digesting amplicon with enzymes Alw I to 48bp and 146bp framents. This method allowed detection of the presence of less than 0.1% deer materials in feedstuffs. It could be one of an effective method for detection deer-derived materials in feedstuff and also an acute method to distignish false and true deer medicine. |
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| Keywords: | Cenus Cervus mitochondrial DNA cytochrome b PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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