| 小鼠2-细胞胚胎细管法和OPS法玻璃化冷冻保存技术的研究 |
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| 引用本文： | 谭鸿明,周崇,洪琼花,杨红远,朱士恩.小鼠2-细胞胚胎细管法和OPS法玻璃化冷冻保存技术的研究[J].中国畜牧杂志,2004,40(5):3-6. |
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| 作者姓名： | 谭鸿明 周崇 洪琼花 杨红远 朱士恩 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100094
2. 云南省畜牧兽医研究所,云南昆明,650224 |
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| 摘 要： | 本试验在室温 (2 0℃和 2 5℃ )条件下 ,利用不同浓度的玻璃化溶液 (EFS和EDFS) ,对小鼠 2 细胞胚胎进行细管法和OPS法玻璃化冷冻保存。在 2 0℃室温条件下 ,用EFS4 0平衡 1min细管一步法冷冻 ,解冻后囊胚发育率仅为35 .0 % ,和新鲜 2 细胞体外培养的对照组 (6 5 .0 % )的差异极显著 (P <0 .0 1)。当 2 细胞胚胎在 10 %EG +10 %D溶液中预处理 5min ,再移入EDFS中平衡 30s二步法冷冻保存 ,解冻后囊胚发育率达 4 7.8%～ 4 8.8% ;当室温升至2 5℃时 ,二步法冷冻保存后 2 细胞的囊胚发育率达到 5 2 .2 % ,与对照组无显著差异 (P >0 .0 5 )。改用OPS二步法EFS30冷冻组保存后的 2 细胞胚胎的囊胚发育率高达 6 2 .2 % ,为试验中的最佳组。用最佳细管法和OPS法冷冻组解冻后培养至囊胚移植给受体母鼠均获得产仔
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| 关 键 词： | 畜牧学 玻璃化冷冻 OPS法 细管法 2细胞胚胎 小鼠 |
| 文章编号： | 0258-7033(2004)05-0003-04 |
| 修稿时间： | 2003年7月1日 |
Study on Cryopreservation of Mouse 2-Cell Embryos by Straw or OPS Vitrification |
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| TAN Hong-ming,ZHOU Chong,HONG qiong-hua,YANG Hong-yuan,ZHU Shi-en.Study on Cryopreservation of Mouse 2-Cell Embryos by Straw or OPS Vitrification[J].Chinese Journal of Animal Science,2004,40(5):3-6. |
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| Authors: | TAN Hong-ming ZHOU Chong HONG qiong-hua YANG Hong-yuan ZHU Shi-en |
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| Institution: | TAN Hong-ming~1,ZHOU Chong~1,HONG qiong-hua~2,YANG Hong-yuan~2,ZHU Shi-en~ |
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| Abstract: | The results of cryopreservation of mouse 2-cell embryos by straw and OPS vitrification method in this study showed that when the 2-cell embryos were equilibrated in EFS40 at 20℃ for 1 min (one-step straw method) before cryopreservation, the blastocysts rate was only 35.0%. There was a significant difference (P<0.01) from the control ((65.0%)). When 2-cell embryos were pretreated in 10% EG+10% D for 5 min then treated in EFS for 30 s (two-step), the blastocysts rates were 47.7%～48.8%. At 25℃, the blastocysts rate for two-step method was 52.2%. There was no significant difference (P>0.05) compared with the control. When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.2%, the highest rate in all groups. After the blastocysts cultured from thawed embryos from the best straw group and OPS group were transferred, all groups had obtained offspring. |
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| Keywords: | Animal science Vitrification OPS method Straw method 2-Cell embryo Mouse |
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