Vitamin C requirement of kuruma shrimp postlarvae, Marsupenaeus japonicus (Bate), using l-ascorbyl-2-monophosphate-Na/Ca |
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| Authors: | Yin Yin Moe Shunsuke Koshio Manabu Ishikawa Shin-ichi Teshima Arthur Panganiban Jr Moe Thu Fady Raafat Michael & Torgjun Ren |
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| Institution: | Science of Marine Resources, The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan; Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Kagoshima, Japan; Department of Fisheries, Ministry of Livestock and Fisheries, Yangon, Myanmar; Institute of Aquaculture, Zamboanga State Colleage of Marine Sciences and Technology, Zamboanga, Philippines |
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| Abstract: | l ‐ascorbyl‐2‐monophosphate‐Na/Ca (AMP‐Na/Ca) was used as a vitamin C source to investigate the ascorbic acid (AsA) requirements on growth performance and stress resistance of the post‐larval kuruma shrimp, Marsupenaeus japonicus. Purified carrageenan‐microbound diets with six levels of AMP‐Na/Ca, AsA equivalent to 0, 20, 56, 87, 759 and 1697 mg kg?1 diet were fed to triplicate groups of M. japonicus (mean initial weight 16±0.3 mg) for 30 days. The diets with AsA 0, 20 and 56 mg kg?1 showed high cumulative mortality after 10 days of feeding. After the 30‐day trial, the shrimp fed these diets had significantly lower survival and weight gain (WG, %) than those fed the 87, 759 and 1697 mg AsA kg?1 diets. Specific growth rate and individual dry weight showed the same pattern as WG (%). There were no significant differences in growth performance among the groups fed the AsA levels at 87, 759 and 1697 mg kg?1 at the termination of feeding trial. Broken‐line regression analysis indicated that 91.8 mg AsA kg?1 in the diet was the optimum for post‐larval shrimp. On the other hand, dietary level of more than 800 mg AsA kg?1 was needed to ensure high resistance to stressful conditions such as osmotic and formalin stressors. |
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| Keywords: | l-ascorbyl-2-monophosphate-Na/Ca (AMP-Na/Ca) kuruma shrimp postlarvae AsA requirement growth stress resistance |
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