| 2个不同来源真姬菇子实体同源性的分子鉴定 |
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| 引用本文： | 黄龙花,夏凤娜,邵满超,张一帆,陈多扬,胡惠萍.2个不同来源真姬菇子实体同源性的分子鉴定[J].中国食用菌,2014(1):34-36. |
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| 作者姓名： | 黄龙花 夏凤娜 邵满超 张一帆 陈多扬 胡惠萍 |
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| 作者单位： | [1]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东省华南应用微生物重点实验室,广东广州510070 [2]广东粤微食用菌技术有限公司,广东广州510070 |
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| 基金项目： | 广东省重大科技专项“食用菌工厂化生产碳减排综合创新技术的研究与示范”（2011A080802017）;广东省科技计划农业攻关项目“广东省食药用菌驯化与利用技术研究”（2012A020100010）. |
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| 摘 要： | 为了研究2个真姬菇子实体的同源性，应用ERIC—PCR图谱分析和ITS序列分析对其进行了详细的研究。ERIC—PCR结果显示2个真姬菇存在条带多样性差异，应为不同品种或不同菌株（品系）；ITS序列测定结果显示2个真姬菇样品仅存在3个碱基差异，属于同一品种。2种分子方法结合分析表明，这两个真姬菇子实体来自同一个品种的不同亚种或不同菌株（品系），拥有独立知识产权。
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| 关 键 词： | 真姬菇 ITS序列 ERIC—PCR 菌株（品系） |
Identification of Hypsizigus marmoreus Fruit Body with Different Origin Based on Molecular Markers |
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| HUANG Long-hua,XIA Feng-na,SHAO Man-chao,ZHANG Yi-fan,CHEN Duo-yang,HU Hui-ping.Identification of Hypsizigus marmoreus Fruit Body with Different Origin Based on Molecular Markers[J].Edible Fungi of China,2014(1):34-36. |
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| Authors: | HUANG Long-hua XIA Feng-na SHAO Man-chao ZHANG Yi-fan CHEN Duo-yang HU Hui-ping |
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| Institution: | 1. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology, South China, Guangzhou Guangdong 510070; 2. Guangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou Guangdong 510070) |
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| Abstract: | Two fruit bodies of Hypsizigus marmoreus which provided by two edible fungus enterprises in Guangdong province were studied in order to clarify the homology of them. Enterbacterial repetive intergenic consensus - PCR （ ERIC - PCR） and internal transcribed spacer （ITS） sequence analysis were used in this study. ERIC -PCR results showed that the amplified bands of ERIC - PCR were high polymorphisms, and two Hypsizigus marmoreus were difference in the diversity, which should be different species or different strain. ITS sequence analysis results suggested that the difference of ITS sequence between the two Hypsizigus mar- moreus were not obvious, and the number of variable sites was 3. They were the same species. Combining with the two kinds of mo-lecular method, we could known that these two Hypsizigus marmoreus fruit body were from the same species but different subspecies or different strain. They should had independent intellectual property rights. |
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| Keywords: | Hypsizigus marmoreus ITS sequences ERIC - PCR Molecular identification |
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