| 侵染百合的李属坏死环斑病毒病（PNRSV）的研究 |
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| 引用本文： | 韩丽娟,刘文洪.侵染百合的李属坏死环斑病毒病（PNRSV）的研究[J].中国农业科学,2007,40(9):1943-1951. |
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| 作者姓名： | 韩丽娟 刘文洪 |
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| 作者单位： | 1. 北京出入境检验检疫局,北京,100025
2. 浙江中医学院,杭州,310053 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 【目的】在分子水平上鉴定百合上发生的李属坏死环斑病毒（Prunus necrotic ringspot virus,PNRSV）【方法】对待测百合种球随机抽样，在温室内种植并观察，长出叶片后，用RNA Extraction Kit (Sangon, China)从幼嫩的叶组织中提取核酸，将反转录出的cDNA用 PNRSV的特异性引物进行PCR扩增，电泳PCR产物得到了450bp的目的片段。低熔点胶回收并克隆至pGEM-T-easy载体测序后进行序列比对分析。【结果】 从百合中所扩增的450bpcDNA的核苷酸序列与27个早期已报道的PNRSV分离物的CP 基因的同源性为84.5%～99.1%。【结论】百合是PNRSV的一新寄主。
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| 关 键 词： | 百合 李属坏死环斑病毒 RT-PCR 检测 |
| 收稿时间： | 2006-7-18 |
| 修稿时间： | 2006-07-17 |
Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurred on Lily |
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| HAN Li-juan,LIU Wen-hong.Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurred on Lily[J].Scientia Agricultura Sinica,2007,40(9):1943-1951. |
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| Authors: | HAN Li-juan LIU Wen-hong |
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| Abstract: | 【Objective】Molecular Identification of Prunus necrotic ringspot virus occurred on Lily. 【Method】Lily corms were randomly selected and grown in greenhouse. Total RNAs were extracted from younger leaves with RNA Extraction Kit (Sangon, China), and RT-PCR amplification of CP cDNA fragments was performed with primer pairs specific for PNRSV. An expected cDNA fragment of about 450bp was harvested and cloned into pGEM-T-easy Vector. Clones were sequenced and compared with reported PNRSV isolates in Gene bank【Result】The nucleotide sequence of the cloned cDNA revealed that 450bp sequence is belong to PNRSV, and has a 84.5%~99.1% homology with 27 reported PNRSV isolates.【Conclusion】Identifying the occurrence of Prunus necrotic ringspot virus on Lily with molecular bio-methods, and confirming that lily is a new nature host of PNRSV. |
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| Keywords: | RT-PCR |
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