| 大肠埃希菌F18菌毛结构亚单位抗原特性的研究 |
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| 引用本文： | 成大荣,朱善元,钱金梅,董丙敏.大肠埃希菌F18菌毛结构亚单位抗原特性的研究[J].动物医学进展,2009,30(2). |
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| 作者姓名： | 成大荣 朱善元 钱金梅 董丙敏 |
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| 作者单位： | 1. 扬州大学兽医学院,江苏扬州,225009
2. 江苏畜牧兽医职业技术学院,江苏泰州,225300 |
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| 基金项目： | 泰州市科技发展计划项目，扬州大学科技创新培育基金 |
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| 摘 要： | 利用已经表达的大肠埃希菌F18ab和F18ac菌毛各结构亚单位(FedA、FedE、FedF)的融合蛋白GST-FedA/ab、GST-FedA/ac、GST-FedE/ab、GST-FedF/ab、GST-FedF/ac分组肌肉注射免疫成年健康家兔,分别制备抗GST-FedA/ab、GST-FedA/ac、GST-FedE、GST-FedF/ab、GST-FedF/ac的多价血清.玻板凝集试验结果表明,抗GST-FedA/ab、GST-FedA/ac、抗GST-FedE/ab、GST-FedF/ab、GST-FedF/ac多价血清均能同时凝集F18ab+大肠埃希菌F107/86株、F18ac+大肠埃希菌8813株.通过荧光抗体染色法对抗FedA/ab、FedA/ac、FedE/ab、FedF/ab、FedF/ac的单因子血清的研究,发现F18菌毛"a"抗原因子分布于FedA、FedE、FedF亚单位上,"b/c"抗原因子分布于FedA、FedF亚单位上.
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| 关 键 词： | 大肠埃希菌 F18菌毛 结构亚单位 抗原因子 |
Study on Antigenic Characteristics of the Subunits of E.coli Fimbria F18 |
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| CHENG Da-rong,ZHU Shan-yuan,QIAN Jin-mei,DONG Bing-min.Study on Antigenic Characteristics of the Subunits of E.coli Fimbria F18[J].Progress In Veterinary Medicine,2009,30(2). |
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| Authors: | CHENG Da-rong ZHU Shan-yuan QIAN Jin-mei DONG Bing-min |
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| Institution: | 1.College of Veterinary Medicine;Yangzhou University;Yangzhou;Jiangsu;225009;China;2.Jiangsu Animal Husbandry and Veterinary College;Taizhou;225300;China |
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| Abstract: | Five kinds of the Escherichia coli fimbria F18 subunit recombinant proteins: GST-FedA/ab,GST-FedA/ac,GST-FedE/ab,GST-FedF/ab and GST-FedF/ac were extracted with SDS-PAGE method.Five groups of rabbits were immunized with the five recombinated proteins respectively,and five kinds of antisera: anti-GST-FedA/ab,anti-GST-FedA/ac,anti-GST-FedE/ab,anti-GST-FedF/ab and anti-GST-FedF/ac were obtained.The results of the direct agglutination test of the five kinds of antisera by using F18ab-positive E.coli strain F107... |
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| Keywords: | Escherichia coli fimbria F18 subunit antigenic factor |
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