Thermal shock induction of triploidy in Atlantic cod (Gadus morhua L.) |
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| Authors: | Stefano Peruzzi Anne Kettunen Raul Primicerio & Goran Kauri&#; |
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| Institution: | Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway; Fiskeriforskning (Norwegian Institute of Fisheries and Aquaculture Research), Tromsø, Norway; Department of Immunology and Transfusion Medicine, University Hospital of North Norway (UNN), Tromsø, Norway |
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| Abstract: | The effects of thermal treatments on induction of triploidy in Atlantic cod have been investigated. Cold shock ?1.7±0.1°C at 20 min post fertilization (PF) for 2 h] was based on a previously developed protocol, and heat shocks, below the lethal threshold of 24°C, were at 16, 18 or 20°C applied 20, 30 or 40 min PF for 20 min. Cold shock did not affect larval survival and was ineffective for producing triploids (range 0–4%). A heat shock of 20°C at 20 min PF generated the highest percentages (range 66–100%) of triploid larvae at hatching, with survival ranging from 10% to 20% relative to the controls. Lower heat shock temperatures or delayed shocks increased survival but decreased the number of triploids, providing no net gain in triploid yield (range 1–9%). Heat shocks applied later than 20 min PF produced 2–4% tetraploid larvae at hatching. A thermal shock of 20°C initiated at 20 min PF and lasting 20 min proved to be the most generally efficient treatment for induction of triploidy in Atlantic cod. |
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| Keywords: | triploidy thermal treatment sterility gadids |
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