| 蓝果树茎段组织培养和快速繁殖 |
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| 引用本文： | 王春荣,毕君,曹书敏.蓝果树茎段组织培养和快速繁殖[J].北方园艺,2011(1):139-143. |
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| 作者姓名： | 王春荣 毕君 曹书敏 |
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| 作者单位： | 河北省林木良种工程技术研究中心,河北石家庄,050061;河北省林业科学研究院,河北石家庄,050061;河北科技师范学院,河北秦皇岛,066004 |
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| 基金项目： | 国家科技支撑计划林业资助项目(2006BAD03A01) |
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| 摘 要： | 以蓝果树带腋芽半木质化茎段为外植体,进行组织培养研究。结果表明:适宜消毒处理为75%酒精30 s+0.1%HgCl28 min;最佳增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.1mg/L;根诱导以1/2MS+NAA 0.5 mg/L为宜,生根率达97.5%。
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| 关 键 词： | 蓝果树 茎段 组织培养 快繁 |
Tissue Culture of Stem Segments and Rapid Propagation of Nyssa sinensis Oliv. |
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| WANG Chun-rong,BI Jun,CAO Shu-min.Tissue Culture of Stem Segments and Rapid Propagation of Nyssa sinensis Oliv.[J].Northern Horticulture,2011(1):139-143. |
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| Authors: | WANG Chun-rong BI Jun CAO Shu-min |
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| Institution: | WANG Chun-rong1,2,BI Jun1,CAO Shu-min3(1.Hebei Engineering Research Center for Trees Varieties,Shijiazhuang,Hebei 050061,2.Hebei Academy of Forestry Science,3.Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004) |
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| Abstract: | Taking the stem-segment with axillary buds as explants in tissue culture.The results showed that the best disinfectant was dip in 75% ethyl alcohol for 30 s,then agitating in a solution of 0.1%HgCl2 for 8 min;The best multiplication medium was MS+6-BA 1.0 mg/L+IBA 0.1 mg/L;The best induction medium for roots was 1/2MS+NAA 0.5 mg/L,rooting rate was 97.5%. |
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| Keywords: | Nyssa sinensi Oliv stem tissue culture rapid propagation |
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