| 铁皮石斛组培苗耐盐性研究 |
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| 引用本文： | 李国树,徐成东,李天星,杜富祥,邱璐,李雪玲.铁皮石斛组培苗耐盐性研究[J].北方园艺,2011(1):153-156. |
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| 作者姓名： | 李国树 徐成东 李天星 杜富祥 邱璐 李雪玲 |
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| 作者单位： | 楚雄师范学院化学与生命科学系,云南楚雄,675000;滇中高原生物资源与利用研究所,云南楚雄,675000;楚雄师范学院化学与生命科学系,云南楚雄,675000 |
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| 基金项目： | 楚雄师范学院学术后备人才资助项目(08YJRC22); 云南省应用基础研究计划资助项目(2008CD218) |
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| 摘 要： | 以铁皮石斛为外植体诱导出愈伤组织后,分别接种到不同盐浓度的愈伤增殖、茎叶分化和生根3种培养基中进行耐盐性筛选,30 d后进行脯氨酸含量测定。结果表明:在培养基中,NaCl浓度在0.2%～1.0%范围内,对铁皮石斛的愈伤增殖、茎叶分化和生根均产生抑制作用,并且盐浓度越高,脯氨酸含量也随之升高,抑制作用越大。盐浓度达0.6%时,愈伤诱导受害明显,脯氨酸含量是对照组的3.60倍;盐浓度高于0.5%时,盐胁迫对不定根和茎叶分化诱导更显著。
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| 关 键 词： | 铁皮石斛 组培苗 耐盐性 临界盐浓度 |
Research of the Salt Resistance in Seedling Cultivation of Dendrobium of ficinale |
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| LI Guo-shu,XU Cheng-dong,LI Tian-xing,DU Fu-xiang,QIU Lu,LI Xue-ling.Research of the Salt Resistance in Seedling Cultivation of Dendrobium of ficinale[J].Northern Horticulture,2011(1):153-156. |
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| Authors: | LI Guo-shu XU Cheng-dong LI Tian-xing DU Fu-xiang QIU Lu LI Xue-ling |
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| Institution: | LI Guo-shu1,2,XU Cheng-dong1,LI Tian-xing1,DU Fu-xiang1,QIU Lu1,Ll Xue-ling1,2(1.Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000,2.Institute for Bio-resources Research and Development of Central Yunnan Plateau,Yunnan 675000) |
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| Abstract: | Took propagated tissue culture of Dendrobium officinale callus as the material.It was inoculated into three different concentration of medium salt tolerance screening.The results showed that 30 days under salt stress,different salt concentration on callus induction of Dendrobium obviously higher than the 0.6% in the salt concentration,tissue culture,significantly affected,proline was the 3.60 times of content;different salt concentration on differentiation of stem and root induction and growth were influent... |
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| Keywords: | Dendrobium officinale seedling cultivation salt resistance critical concentration |
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