| 虎舌红种内花粉形态与活力的比较研究 |
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| 引用本文： | 杜娟,邵慧敏,叶萌.虎舌红种内花粉形态与活力的比较研究[J].北方园艺,2011(1):80-83. |
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| 作者姓名： | 杜娟 邵慧敏 叶萌 |
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| 作者单位： | 四川农业大学城乡建设学院,四川,都江堰,611830;中科院华西植物园,四川,都江堰,611830;四川农业大学林学院,四川,雅安,625000 |
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| 摘 要： | 通过扫描电镜观察了虎舌红及其2个新品种红虎舌和绿虎舌的花粉形态特征,并用TTC染色法和离体培养法测定其花粉的活力,比较了虎舌红种内花粉形态及活力的差异,为虎舌红的杂交育种等进一步的开发利用提供依据。结果表明:3种花粉均为N3P4C5型,单粒,等极,辐射对称,椭球形,极面观三裂圆形或三角形,赤道面观椭圆形,萌发孔为3孔沟,沟纵长,直达两极,大部分形成合沟;外壁纹饰为虎舌红呈穴网状,红虎舌呈颗粒状,绿虎舌呈网纹状,依据3种花粉的大小和外壁纹饰的差异,推断出红虎舌的性状较为原始,而绿虎舌的性状较为进化;用TTC染色法和离体培养法对3种花粉的活力大小进行了比较,其结果一致,均为红虎舌>绿虎舌>虎舌红,3种花粉最佳的贮藏方式均为在4℃条件下贮藏。
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| 关 键 词： | 虎舌红 花粉形态 花粉活力 扫描电镜 TTC染色法 |
Analysis of Pollen Shape and Livingness in Ardisia mamillata |
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| DU Juan,SHAO Hui-min,YE Meng.Analysis of Pollen Shape and Livingness in Ardisia mamillata[J].Northern Horticulture,2011(1):80-83. |
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| Authors: | DU Juan SHAO Hui-min YE Meng |
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| Institution: | DU Juan1,SHAO Hui-min2,YE Meng3(1.City Construction College of Sichuan Agricultura University,Dujiangyan,Sichuan 611830,2.Western China Arboretum of the Chinese Academy of Sciences,3.Forestry College of Sichuan Agriculture University,Ya'an,Sichuan 625000) |
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| Abstract: | In order to compare the difference of pollen shape and livingness in Ardisia mamillata,the pollen of the plant were cultured in vitro and then its shape were observed through scanning electronic microscope(SEM),as well as the pollen livingness were measured by TTC.The results showed that the pollen type was N3P4C5,single,radiation symmetry,ellipse sphericity,;the polar cover was triangle or three crack rotundity,the equator cover was ellipse,the bourgeon aperture was three.every aperture from one polar to a... |
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| Keywords: | Ardisia mamillata pollen shape pollen livingness scanning electronic microscope TTC coloration |
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