Molecular epidemiological investigation of Brucella melitensis circulating in Mongolia by MLVA16 |
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| Institution: | 1. OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea;2. Institute of Veterinary Medicine, Zaisan, 17024, Ulaanbaatar, Mongolia;3. Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Republic of Korea;1. Centre de Recerca en Sanitat Animal (CReSA) - Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, Barcelona, Spain;2. Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Barcelona, Spain;3. Departament of Animal Science, University of Lleida, Lleida, Spain;4. Agrotecnio Centre, University of Lleida, Lleida, Spain;5. Transfer in Bovine Reproduction SLu, 22300 Barbastro, Spain;1. Veterinary Epidemiology, Economics and Public Health Group, Department of Production and Population Health, The Royal Veterinary College, University of London, North Mymms, Hertfordshire AL9 7TA, United Kingdom;2. Faculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, Keppel Street, Bloomsbury, London WC1E 7HT, United Kingdom;3. Ministry of Agriculture, Zarqa Agriculture Department, Zarqa, Jordan |
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| Abstract: | Mongolia has a high incidence of brucellosis in human and animals due to livestock husbandry. To investigate the genetic characteristics of Mongolian B. melitensis, an MLVA (multi-locus variable-number tandem-repeat analysis)-16 assay was performed with 94 B. melitensis isolates. They were identified as B. melitensis biovar (bv.) 1 (67), 3 (10) and Rev. 1 vaccine strains (17) using a classical biotyping and multiplex PCR. In genotyping, three human isolates were grouped at 2 genotypes with sheep isolates, and it implies that B. melitensis are cross-infected between human and livestock. In the parsimony analysis, Mongolian B. melitensis isolates had high genetic similarity with Chinese strains, likely due to the geographical proximity, clustered distinctively as compared with other foreign isolates.B. melitensis Rev. 1 vaccine strains were divided into 4 genotypes with 92% similarity. In the analysis of Rev.1 strains, the risk of mutation of vaccine strain might not be overlooked. Animal quarantines should be strengthened to prevent the spread of Brucella species among adjacent countries. |
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| Keywords: | Epidemiology MLVA-16 Mongolia China Zoonoses |
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