Quantification of DNA of citrus huanglongbing pathogen in diseased leaves using competitive PCR |
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| Authors: | Kunimasa Kawabe Nguyen Thi Ngoc Truc Bui Thi Ngoc Lan Le Thi Thu Hong Masatoshi Onuki |
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| Institution: | (1) Japan International Research Center for Agriculture Science (JIRCAS), Tropical Agriculture Research Front, Okinawa 907-0002, Japan;(2) Southern Fruit Research Institute (SOFRI), Tien Giang, Vietnam |
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| Abstract: | A quantification system for huanglongbing pathogen using a competitive polymerase chain reaction method and image-analyzing
software were developed to obtain precise results. Significant differences in the quantity of pathogen were thus determined
in leaves of two citrus cultivars commonly cultivated in southern Vietnam. Less pathogen-related DNA was detected from the
tissue of citrus cultivars that are believed to be more tolerant than susceptible cultivars. The quantification system will
be used in studies on pathogen proliferation and movement inside citrus tissue. |
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| Keywords: | Huanglongbing Citrus greening Candidatus Liberibacter asiaticus Competitive PCR Quantification |
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