| 饲料中阿散酸的高效液相色谱检测技术的研究 |
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| 引用本文： | 王金荣,张丽英,李德发.饲料中阿散酸的高效液相色谱检测技术的研究[J].中国畜牧杂志,2008,44(7):34-37. |
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| 作者姓名： | 王金荣 张丽英 李德发 |
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| 作者单位： | 1. 农业部饲料效价与安全监督检验测试中心(北京),北京,100094;河南工业大学生物工程学院,河南,郑州,450001
2. 农业部饲料效价与安全监督检验测试中心(北京),北京,100094 |
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| 摘 要： | 建立了饲料中阿散酸的高效液相色谱-离子交换分离-紫外检测的定量分析方法。用25mmol/L的氢氧化钠溶液作为提取剂,在50℃水浴中提取30min,以40mmol/L磷酸氢二钠-甲醇(960∶40,V/V)为流动相等度洗脱,在阴离子色谱分离柱上进行分离,244nm处检测,12min内分离阿散酸。饲料中阿散酸的平均回收率达到92.4%～99.6%,相对标准偏差小于4.9%。阿散酸的检出限为0.2mg/L,定量限为1mg/kg。
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| 关 键 词： | 阿散酸 液相色谱 离子交换 饲料 |
| 文章编号： | 0258-7033(2008)07-0034-04 |
| 修稿时间： | 2007年3月5日 |
Determination of Arsanilic acid in Feeds by High Performance Liquid Chromatogram |
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| WANG Jin-rong,ZHANG Li-ying,LI De-fa.Determination of Arsanilic acid in Feeds by High Performance Liquid Chromatogram[J].Chinese Journal of Animal Science,2008,44(7):34-37. |
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| Authors: | WANG Jin-rong ZHANG Li-ying LI De-fa |
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| Abstract: | A HPLC method with anion exchange for the determination of arsanilic acid in feeds was developed. The extracted solution was 25 mmol/L sodium hydroxide solution. The analyte was eluted on anion exchange column with ultraviolet detection at 244 nm. 40 mmol/L sodium dihydrogen phosphate-methanol (96∶4,V/V) solution was chosen as the mobile phase. The average recoveries of arsanilic acid in feeds were 92.4%～99.6%. The limit of detection was 0.2 mg/L and the limit of quantification was 1 mg/kg. |
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| Keywords: | arsanilic acid HPLC anion exchange feed |
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