| 氨基糖苷类耐药鸭大肠杆菌acrD基因mRNA表达水平 |
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| 引用本文： | 刘建华,苑丽,潘玉善,陈玉霞,胡功政,陈永杰.氨基糖苷类耐药鸭大肠杆菌acrD基因mRNA表达水平[J].中国兽医学报,2011,31(6). |
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| 作者姓名： | 刘建华 苑丽 潘玉善 陈玉霞 胡功政 陈永杰 |
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| 作者单位： | 1. 河南农业大学,牧医工程学院,河南郑州450002
2. 河南省新郑市农业局,河南新郑,451100 |
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| 基金项目： | 国家自然科学基金资助项目(31072170) |
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| 摘 要： | 从89株临床分离鸭源大肠杆菌中选择5株氨基糖苷类高水平耐药菌,应用实时荧光定量RT-PCR方法测定其主动外排基因acrD mRNA水平,选用1株中介株、1株敏感株和大肠杆菌ATCC25922作为对照,分析其mRNA转录水平与耐药强度的相关性。结果显示,5株耐药菌的acrD mRNA相对表达量均高于中介株和敏感株,其相对表达量分别是2.00、1.96、2.22、1.84、4.39,敏感对照株为1.67,中介对照株为1.75,除4号菌株外,其余4株耐药菌相对表达量和标准菌株ATCC25922具有显著性差异;同是高度耐药的4号菌和5号菌存在较大差异,5号菌相对表达量是4号菌的2.39倍,这说明对氨基糖苷类不同耐药程度的菌株acrD基因的转录、表达存在差异,且与耐药水平呈正相关,但同时存在其他重要耐药机制介导氨基糖苷类耐药。
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| 关 键 词： | 鸭大肠杆菌 实时荧光定量RT-PCR 氨基糖苷类耐药性 acrD |
The expression of acrD gene of aminoglycosides-resistant enteropathogenic E.coli isolated from duckling |
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| LIU Jian-hua,YUAN Li,PAN Yu-shan,CHEN Yu-xia,HU Gong-zheng,CHEN Yong-jie.The expression of acrD gene of aminoglycosides-resistant enteropathogenic E.coli isolated from duckling[J].Chinese Journal of Veterinary Science,2011,31(6). |
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| Authors: | LIU Jian-hua YUAN Li PAN Yu-shan CHEN Yu-xia HU Gong-zheng CHEN Yong-jie |
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| Institution: | LIU Jian-hua1,YUAN Li1,PAN Yu-shan1,CHEN Yu-xia1,HU Gong-zheng1,CHEN Yong-jie2(1.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China,2.Xinzheng Municipal Bureau of Agriculture of Henan Province,Xinzheng,Henan 451100,China) |
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| Abstract: | Five aminoglycoside drug-resistance strains were selected from 89 clinical strains,to detect the expression of acrD gene by real-time RT-PCR,1 midrange drug-resistance strain,1 sensitive strain and ATCC25922 as control strains.The relative levels of acrD gene expression of drug-resistance strains was higher than the midrange drug-resistance strain,the sensitive strain and ATCC25922.The relative levels of acrD gene expression of five drug-resistance strains were 2.00,1.96,2.22,1.84 and 4.39,whereas it was 1.... |
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| Keywords: | Escherichia coli from duckling real-time RT-PCR drug resistance acrD |
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