| 鸭毛囊中ASIP基因片段的克隆及组织表达分析 |
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| 引用本文： | 梁正翠,杨海明,王志跃,陈宽维,刘桂琼.鸭毛囊中ASIP基因片段的克隆及组织表达分析[J].中国兽医学报,2011,31(6). |
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| 作者姓名： | 梁正翠 杨海明 王志跃 陈宽维 刘桂琼 |
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| 作者单位： | 1. 扬州大学,动物科学与技术学院,江苏扬州225009
2. 中国农业科学院家禽科学研究所,江苏扬州,225003 |
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| 基金项目： | 国家科技支撑计划资助项目(2006BDA01A09) |
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| 摘 要： | 根据GenBank发表的鸡刺鼠信号蛋白(ASIP)基因序列的同源保守区域,设计了1对特异性引物。以番鸭、高邮鸭和英系北京鸭(即樱桃谷鸭)毛囊RNA为模板,经扩增、测序及拼接得到的部分mRNA序列均为编码序列,共308bp,对应编码102个氨基酸。与已发表的原鸡和鹌鹑的ASIP对应的mRNA核苷酸序列进行比对,番鸭、高邮鸭和英系北京鸭的同源性均在92.53%以上,相应编码的氨基酸序列同源性均高于92.16%。高邮鸭与英系北京鸭核苷酸,氨基酸序列一致,同源性为100%。半定量PCR结果显示,番鸭ASIP基因表达具有较高的普遍性,在皮肤组织(毛囊和皮肤)和中枢神经系统(下丘脑)及其他非皮肤中均有表达。皮肤和毛囊的表达差异不显著(P>0.05),下丘脑中的ASIP基因相对表达量最高,极显著高于皮肤中ASIP基因的表达量(P<0.01),而与毛囊中的差异不显著(P>0.05)。
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| 关 键 词： | 鸭 刺鼠信号蛋白基因 克隆 组织表达 |
Cloning of agouti signaling protein gene in ducks' hair follicles and its tissue expression |
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| LIANG Zheng-cui,YANG Hai-ming,WANG Zhi-yue,CHEN Kuan-wei,LIU Gui-qiong.Cloning of agouti signaling protein gene in ducks' hair follicles and its tissue expression[J].Chinese Journal of Veterinary Science,2011,31(6). |
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| Authors: | LIANG Zheng-cui YANG Hai-ming WANG Zhi-yue CHEN Kuan-wei LIU Gui-qiong |
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| Institution: | LIANG Zheng-cui1,YANG Hai-ming1,WANG Zhi-yue1,CHEN Kuan-wei2,LIU Gui-qiong1(1.College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China,2.Poultry lnstitute,Chinese Academy of Agricultural Sciences,Jiangsu 225003,China) |
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| Abstract: | A pair of specificity primer was designed for amplification of ASIP gene in ducks(Muscovy duck,Gaoyou duck and the British strain of Pekin-duck) according to the known sequence of chicken of GenBank.The fragment of ASIP gene in ducks was obtained by PCR amplification and splicing from the extracted total RNA of hair follicle.The complete sequence was 308 bp cds and codes 102 amino acids.Compared with the chicken and quail corresponding mRNA sequences of ASIP gene in GenBank,the homologies of the nucleotide ... |
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| Keywords: | ducks asip gene cloning tissue expression |
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