| 蛋用型祖代鸡群禽白血病病毒感染状态的持续观察 |
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| 引用本文： | 李中明,王景艳,张青婵,赵冬敏,崔治中.蛋用型祖代鸡群禽白血病病毒感染状态的持续观察[J].中国兽医学报,2011,31(6). |
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| 作者姓名： | 李中明 王景艳 张青婵 赵冬敏 崔治中 |
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| 作者单位： | 山东农业大学,动物科技学院,山东泰安271018 |
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| 基金项目： | 国家农业公益性行业科研专项经费资助项目(200803019) |
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| 摘 要： | 为了检测从国外直接进口的蛋用型祖代鸡群是否存在外源性禽白血病病毒(ALV)的感染,将240只1日龄鸡饲养在严格的SPF环境中。在不同日龄采集泄殖腔棉拭子检测ALV群特异性p27抗原、采集血浆分离外源性ALV和采集血清检测ALV-AB及J特异性抗体。结果表明:在近3个月的6次采样检测中,6个不同的配套系间泄殖腔棉拭子检出率显著不同。其中1个配套系6次完全阴性,其余5个则在不同时期呈间隙性阳性。各品系之间ALV p27抗原检出率与快慢羽性状没有相关性。慢羽的配套系C,36只鸡中在45日龄检出了1例ALV-AB抗体一过性阳性,在60日龄检出了2例ALV-J抗体一过性阳性。分别在5、21日龄采血浆在DF1细胞分离病毒,所有的6个配套系的204只鸡外源性ALV的病毒分离均为阴性。
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| 关 键 词： | 蛋用型祖代鸡 禽白血病病毒 感染状态 检测 |
Long-term inspection on imported egg-type grandparent breeder chicken for avian leukosis virus infection status |
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| LI Zhong-ming,WANG Jing-yan,ZHANG Qing-chan,ZHAO Dong-min,CUI Zhi-zhong.Long-term inspection on imported egg-type grandparent breeder chicken for avian leukosis virus infection status[J].Chinese Journal of Veterinary Science,2011,31(6). |
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| Authors: | LI Zhong-ming WANG Jing-yan ZHANG Qing-chan ZHAO Dong-min CUI Zhi-zhong |
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| Abstract: | To inspect imported egg-type grandparent breeder chicks for their avian leukosis virus(ALV) infection status.Two hundred and forty 1-day-old chicks were kept and raised in SPF isolators.The cloaca swabs,blood plasmas and sera were collected for 6 times at different ages during about 3 months.They were tested for p27 antigen detection,virus isolation and antibodies.The results indicated that the positive rates of p27 antigen were quite different among 6 varieties of the grandparent breeders.One of them were ... |
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| Keywords: | egg-type grandparent breeder chickens avian leukosis virus infection status inspection |
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