| 苹果果实中苹果酸代谢关键酶与苹果酸和可溶性糖积累的关系 |
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| 引用本文： | 姚玉新,李明,由春香,刘志,王冬梅,郝玉金.苹果果实中苹果酸代谢关键酶与苹果酸和可溶性糖积累的关系[J].园艺学报,2010,37(1):1-6. |
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| 作者姓名： | 姚玉新 李明 由春香 刘志 王冬梅 郝玉金 |
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| 作者单位： | (山东农业大学园艺科学与工程学院, 国家苹果工程技术研究中心, 作物生物学国家重点实验室, 山东泰安271018; 辽宁省果树科学研究所, 辽宁熊岳115009) |
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| 基金项目： | 国家自然科学基金青年基金项目(30700549) |
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| 摘 要： | 以‘东光’ב富士’苹果杂交后代为试材,研究苹果果实中苹果酸代谢关键酶与苹果酸和可溶性糖积累的关系。结果表明,负责苹果酸合成的磷酸烯醇式丙酮酸羧化酶(PEPC)、细胞质NAD依赖的苹果酸脱氢酶(cyMDH)和负责苹果酸跨膜运输的H+-焦磷酸酶(VHP)在高、低酸苹果果实成熟过程中活性没有明显差异,而负责苹果酸跨膜运输的H+-ATPase酶(VHA)在高酸果实中有较高的活性,负责苹果酸降解的细胞质NADP依赖的苹果酸酶(cyME)和ATP依赖的磷酸烯醇式丙酮酸羧化激酶(PEP-CK)则在低酸果实中有较高的活性。还从苹果酸向可溶性糖转化的角度探讨了苹果酸代谢关键酶对可溶性糖积累的影响。
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| 关 键 词： | 苹果 苹果酸代谢关键酶 糖 酸积累 |
| 收稿时间： | 2009-7-28 |
| 修稿时间： | 2009-11-4 |
Relationship Between Malic Acid Metabolism-related Key Enzymes and Accumulation of Malic Acid as Well as the Soluble Sugars in Apple Fruit |
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| YAO Yu-xin,LI Ming,YOU Chun-xiang,LIU Zhi,WANG Dong-mei,HAO Yu-jin.Relationship Between Malic Acid Metabolism-related Key Enzymes and Accumulation of Malic Acid as Well as the Soluble Sugars in Apple Fruit[J].Acta Horticulturae Sinica,2010,37(1):1-6. |
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| Authors: | YAO Yu-xin LI Ming YOU Chun-xiang LIU Zhi WANG Dong-mei HAO Yu-jin |
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| Institution: | 1.Department of Pathophysiology and Hgih Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, 2Key Laboratory of High Altitude Medicine, Ministry of Education, 3The Key Laboratory of High Altitude Physiology and High Altitude Disease, PLA, 4Department of High Altitude Disease, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China. E-mail: gyq@mail.tmmu.com.cn |
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| Abstract: | The relationship between activity of malic acid metabolism-related key enzymes and accumulation of malic acid as well as the soluble sugars was investigated by the hybrid from the crossing between apple cultivar 'Dongguang' and 'Fuji'.The results showed that cytosolic phosphoenolpyruvate carboxylase(PEPC)and NAD-dependent malate dehydrogenase(cyMDH),implicated in malic acid(MA)synthesis in the cytosol,and vacuolar H+-pyrophosphatase(VHP),implicated in MA storage in the vacuole,exhibited no significant diffe... |
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| Keywords: | apple key enzymes involved in malic acid metabolism accumulations of malic acid and the soluble sugars |
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