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天葵提取物抗TMV生物活性初探
引用本文:李明,毕亚玲,Sopone Wongkaew.天葵提取物抗TMV生物活性初探[J].贵州大学学报(农业与生物科学版),2008,27(5).
作者姓名:李明  毕亚玲  Sopone Wongkaew
作者单位:1. 贵州大学,作物保护研究所,贵州,贵阳,550025
2. 安徽科技学院,安徽,凤阳,233100
3. Suranaree University of Technology, Thailand Nakhon Ratchasima 30000
基金项目:贵州省国际科技合作重点项目资助(黔科合国字2003014)
摘    要:用系统溶剂提取法得到天葵的石油醚、氯仿、乙酸乙酯、丙酮、乙醇、甲醇和水提取物,天葵提取物的总提取率为38.76%。在烟叶半叶枯斑法活性测定离体试验中,首次发现天葵块根的7种不同溶剂提取物都具有较好的抗TMV生物活性,天葵提取物对TMV钝化作用范围为34.12%~68.09%,其中以天葵的石油醚、丙酮和乙醇提取物效果较好,钝化效果分别为68.09%、63.28%和60.59%;保护作用范围为17.48%~65.26%,以石油醚提取物保护作用最好,抑制率达65.26%;治疗作用范围为12.79%~34.24%,丙酮提取物治疗抑制率可达34.24%.

关 键 词:天葵  提取物  TMV  生物活性

A preliminary study on bioactivity of extracts of Semiaquilegia adoxoides (DC.) Makino against tobacco mosaic virus (TMV)
LI Ming,BI Ya-ling,Sopone Wongkaew.A preliminary study on bioactivity of extracts of Semiaquilegia adoxoides (DC.) Makino against tobacco mosaic virus (TMV)[J].Journal of Mountain Agriculture & Biology,2008,27(5).
Authors:LI Ming  BI Ya-ling  Sopone Wongkaew
Abstract:In this study,petroleum ether,chloroform,ethyl acetate,acetone,ethanol,methanol and water extracts of S.adoxoides were obtained by adopting systemic solvent extraction method.The total extraction percentage of S.adoxoides extract was 38.76 %.In vitro experiment,all solvent extracts of S.adoxoides root were found to have the anti-TMV activity for the first time,and their passivation action range was 34.12%-68.09%.Among these seven extracts,extracts of petroleum ether,acetone and ethanol to TMV had more passi...
Keywords:Semiaquilegia adoxoides  extracts  TMV  bioactivity  
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