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产后患子宫内膜炎对奶牛血清和子宫黏液中免疫球蛋白质量浓度的影响
引用本文:那立冬,张世栋,董书伟,闫宝琪,杨洪早,桑梦琪,贾 宁,王东升,严作廷.产后患子宫内膜炎对奶牛血清和子宫黏液中免疫球蛋白质量浓度的影响[J].西北农业学报,2017(4):503-508.
作者姓名:那立冬  张世栋  董书伟  闫宝琪  杨洪早  桑梦琪  贾 宁  王东升  严作廷
作者单位:(1. 甘肃农业大学 动物医学院,兰州 730070;2. 中国农业科学院 兰州畜牧与兽药研究所,兰州 730050)
基金项目:中央级公益性科研院所基本科研业务费专项(1610322015003);“十二五”国家科技支撑计划(2012BAD12B03);中国农业科学院农业科技创新工程(CAAS-ASTIP-2014-LIHPS-03)。
摘    要:为比较分娩后患子宫内膜炎奶牛和健康奶牛血清及子宫黏液中IgA、IgM和IgG的变化,采用酶联免疫吸附测定法分别对奶牛血清和子宫黏液中IgA、IgM和IgG的质量浓度进行定量检测。结果表明,患病奶牛血清中的IgA和健康奶牛子宫黏液中的IgA持续降低,子宫黏液中IgA先降低后升高,健康奶牛血清中IgA变化不大;患病奶牛和健康奶牛子宫黏液和血清中IgG均先降低后升高;健康奶牛血清和子宫黏液中IgM先降低后升高,患病奶牛子宫黏液中IgM先升高后降低,后又升高,患病奶牛血清中IgM逐渐降低;患病奶牛血清中IgG始终高于健康奶牛的,说明IgG在全身体液免疫中发挥主要作用;患病奶牛子宫黏液的IgA和IgM高于健康奶牛,说明IgA和IgM在子宫局部免疫中发挥主要作用。

关 键 词:奶牛  子宫内膜炎  免疫球蛋白  质量浓度

Effects of Dairy Cows with Endometritis on Immunoglobulin Mass Concentration in Serum and Uterus Mucus of Postpartum
NA Lidong,ZHANG Shidong,DONG Shuwei,YAN Baoqi,YANG Hongzao,SANG Mengqi,JIA Ning,WANG Dongsheng and YAN Zuoting.Effects of Dairy Cows with Endometritis on Immunoglobulin Mass Concentration in Serum and Uterus Mucus of Postpartum[J].Acta Agriculturae Boreali-occidentalis Sinica,2017(4):503-508.
Authors:NA Lidong  ZHANG Shidong  DONG Shuwei  YAN Baoqi  YANG Hongzao  SANG Mengqi  JIA Ning  WANG Dongsheng and YAN Zuoting
Abstract:In order to compare healthy cows with the mass concentration variation of IgA,IgM and IgG in serum and uterus mucus in postpartum dairy cows sufering from endometritis.The mass concentration of IgA,IgM and IgG in uterus mucus and serum were detected by the method of enzyme-linked immunosorbent assay. The results showed that the mass concentration of IgA reduced continuously both serum of endometritis cows and uterus mucus of healthy cows. The IgA mass concentration in uterus mucus decreased initially and then increased in cows suffering from endometritis,There was no great change in the mass concentration of IgA in the serum of healthy cows ; IgG levels of the uterus mucus and serum in endometritis cows and healthy cows decreased firstly and then increased; IgM mass concentration in serum and uterus mucus of healthy cows decreased firstly and then increased,IgM mass concentration in uterus mucus of cows with endometritis increased firstly and then decreased,the IgM levels in serum of endometritis cows gradually reduced. These indicated that IgG in serum of cows suffering from endometritis was always higher than that of healthy cows,so it is appoved that IgG played a major role in systemic humoral immunity. The mass concentration of IgA and IgM in uterus mucus of postpartum dairy cows with endometritis was higher than that of healthy cows,this results indicated that IgA and IgM played the key role in local immunity of the uterus.
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