| 茄类内生菌的分离及拮抗细菌的筛选 |
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| 引用本文： | 刘杰凤,韩寒冰,张进凤,罗添喜,詹加钦.茄类内生菌的分离及拮抗细菌的筛选[J].安徽农业科学,2009,37(3):1160-1162. |
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| 作者姓名： | 刘杰凤 韩寒冰 张进凤 罗添喜 詹加钦 |
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| 作者单位： | 茂名学院化学与生命科学学院,广东茂名,525000 |
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| 摘 要： | 目的]分离番茄、辣椒和茄子3种茄科果蔬的内生菌，并对青枯病拮抗细菌进行筛选与鉴定。方法]采用研磨液培养法，利用选择培养基对3种植株进行内生菌分离，并采用平板划线法纯化，及菌块对峙培养法对已纯化的内生菌进行筛选。根据分离菌的培养特性及菌体形态特征、革兰氏染色及生化反应等，对筛选出来的内生拮抗细菌进行鉴定归属。结果]3种植株内生菌分离，共得到53株内生细菌、53株内生真菌、44株内生放线菌。筛选出14株青枯病菌有强拮抗性的内生细菌，分属为芽孢杆菌属、埃希氏菌属、克雷伯氏菌属、土壤单胞菌属、欧文氏菌属、短小杆菌属，其中芽孢杆菌为优势种群，而且芽孢杆菌属菌株对青枯病菌的拮抗作用最强。结论]提供了探索生物防治茄科植物青枯病的有效途径。
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| 关 键 词： | 内生菌 分离 筛选 拮抗作用 |
Isolation of Endophyte and Screening for Antagonistic Bacteria in Solanaceae |
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| Institution: | LIU Jie-feng et ai (College of Chemistry and Life Science, Maoming University, Maoming, Guangdong 525000) |
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| Abstract: | Objective] The aim of this study was to isolate the endophyte of three Solanaceae fruits and vegetables such as tomato,pepper and eggplant,to screen and identify the bacterial wilt antagonistic bacteria.Method] According to the lapping liquid culture method,the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method,so the purified endophyte was screened by the hyphal pieces confront culture method.Furthermore,the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus,Gram stain as well as physiological and biochemical reactions.Result] Fifty-three endophytic bacteria,fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants.The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus,Escherichia,Klebsiella,Agromonas,Erwinia and Curtobacterium respectively.Especially,Bacillus was the dominant species,and Bacillus had the strongest antagonistic effect on the bacterial wilt.Conclusion] This study provides an effective way for biological control of the bacterial wilt in Solanaceae. |
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| Keywords: | Endophyte Isolation Screening Antagonism |
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