| 金针菇多糖的提取及含量测定 |
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| 引用本文： | 徐亚杰,宁波,王磊,张锦玉,逯家富.金针菇多糖的提取及含量测定[J].农产品加工．学刊,2007(9):70-71. |
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| 作者姓名： | 徐亚杰 宁波 王磊 张锦玉 逯家富 |
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| 作者单位： | 长春职业技术学院,吉林,长春,130033 |
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| 摘 要： | 探讨了用温度70℃热水提取、sevag法除蛋白、乙醇分级沉淀提取金针菇多糖,以及用硫酸-蒽酮法测定其含量的方法。该方法测得多糖的总收率为金针菇湿质量的0.11%,测定多糖的线性范围为1～16μg/mL,对金针菇多糖含量测得的RSD小于2.24%,回收率为97.6%～102.8%。
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| 关 键 词： | 硫酸-蒽酮法 金针菇多糖 提取 含量测定 |
| 文章编号： | 1671-9646（2007）09-0070-02 |
| 修稿时间： | 2007-06-29 |
Extraction and Content Determination of Polysaccharide from Flammulina Velutipes |
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| Xu Yajie,Ning Bo,Wang Lei,Zhang Jinyu,Lu Jiafu.Extraction and Content Determination of Polysaccharide from Flammulina Velutipes[J].Nongchanpin Jlagong.Xuekan,2007(9):70-71. |
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| Authors: | Xu Yajie Ning Bo Wang Lei Zhang Jinyu Lu Jiafu |
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| Institution: | Changchun Vocational Institute of Technology College, Changchun 130033, China |
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| Abstract: | This paper reports the polysaccharide extraction and determination from flammunlina velutipes,the process is:extract the polysaccharides from flammunlina velutipes by 70 ℃ water at first,then deproteinize according to the method of Sevag,and then grading precipitate by ethanol.Quantitatively determine the polysaccharides content by anthrone-sulfuric acid method.In this method,the total yield up to 0.11%,the linearly range is 1～16 μg/mL,the RSD is not more than 2.24%,and the range of recovery is 97.6～102.8%. |
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| Keywords: | anthrone-sulfuric acid method polysaccharides flammunlina velutipes extraction |
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