| 山葡萄“双优”再生系统建立的研究 |
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| 引用本文： | 刘洋,吴学彦,李海燕.山葡萄“双优”再生系统建立的研究[J].农产品加工．学刊,2007(8):4-6,9. |
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| 作者姓名： | 刘洋 吴学彦 李海燕 |
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| 作者单位： | 1. 长春职业技术学院,吉林,长春,130033
2. 吉林农业大学,吉林,长春,130118 |
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| 基金项目： | 国家自然科学基金,吉林省杰出青年科学基金,吉林省科学与技术发展计划项目 |
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| 摘 要： | 以山葡萄"双优"茎段、叶柄和叶片为材料,采用不同的激素浓度进行再生体系的建立。结果表明,以山葡萄茎段为外植体,MS为培养基,激素浓度2.0 mg/L的BA和0.02～0.05 mg/L的IBA再生,获得了山葡萄再生芽,分化率达到6.67%～8.36%,激素浓度为0.2mg/L的IBA进行生根培养,经过15～20 d培养,生根率可达100%,获得了山葡萄再生植株。
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| 关 键 词： | 山葡萄 再生 茎段 叶柄 |
| 文章编号： | 1671-9646（2007）08-0004-03 |
| 修稿时间： | 2007-06-06 |
Study on Organogenesis Regeneration System of Vitis Amurensis |
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| Liu Yang,Wu Xueyan,Li Haiyan.Study on Organogenesis Regeneration System of Vitis Amurensis[J].Nongchanpin Jlagong.Xuekan,2007(8):4-6,9. |
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| Authors: | Liu Yang Wu Xueyan Li Haiyan |
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| Institution: | 1.Changchun Vocational Institute of Technology,Changchun,Jilin 130033,China; 2.Jilin Agricultural University,Changchun,Jilin 130118,China |
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| Abstract: | Tissue culture of Vitis Amurensis including of stem-segement and petiole,Leaf,it is important to istablish regeneration system for genetically transformation of Vitis Amurensis by manipulating hormone levels,different explants for Stem-segement were cultured on MS medium supplemented with BA 2.0 mg/L and IBA 0.02~0.05 mg/L,adventitious buds arose directly and the differentiation frequency was about 6.67%~8.36%,concentration of hormone IBA 0.2 mg/L for 15~ 20 days,the rooting rate was about 100%.Restablishing regenerated system of Vitis Amurensis. |
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| Keywords: | Vitis Amurensis regeneration stem-segement petiole |
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