| 解磷根瘤菌液体培养基类型,浓度及透气条件的比较 |
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| 引用本文： | 李剑峰,张淑卿,师尚礼,霍平慧.解磷根瘤菌液体培养基类型,浓度及透气条件的比较[J].草原与草坪,2010,30(1):28-32. |
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| 作者姓名： | 李剑峰 张淑卿 师尚礼 霍平慧 |
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| 作者单位： | 甘肃农业大学,草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃,兰州,730070 |
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| 基金项目： | 国家科技部“西北优势和特色牧草生产加工关键技术研究与示范”(2007BAD52B06);;国家科技部“奶牛优质饲草生产技术研究及开发”(2006BAD04A04);;国家科技部“优质抗逆专用草新品种选育”(2006BAD01A19);;农业部行业专项“人工草地优质牧草生产动手术研究与示范”(nyhyzx07-022)资助 |
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| 摘 要： | 以解磷根瘤菌SL01为供试菌株,采用液体培养研究了培养基类型和浓度以及透气状况对其生长的影响。结果表明:供试的培养基中,最适的液体种子培养基为LB和1/2 LB液体培养基,菌株的繁殖速度不仅与培养基的类型有关,更受到营养条件的限制;培养基接入解磷根瘤菌SL01并充分发酵之后,发酵液的pH值都相当程度的偏离了初始的酸碱度;根瘤菌作为好气菌,YMA液体培养基中培养12 h后,透气条件下发酵液内的活菌数达12.2×109cfu/mL,为密闭培养条件下的10.51倍,在解磷根瘤菌的发酵培养中,透气状况应作为重要的环节进行考虑。
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| 关 键 词： | 根瘤菌 培养基 透气条件 |
Ventilation conditions on phosphorus-dissolving Rhizobia |
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| LI Jian-feng,ZHANG Shu-qing,SHI Shang-li,HUO Ping-hui.Ventilation conditions on phosphorus-dissolving Rhizobia[J].Grassland and Turf,2010,30(1):28-32. |
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| Authors: | LI Jian-feng ZHANG Shu-qing SHI Shang-li HUO Ping-hui |
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| Institution: | College of Grassland Science;Gansu Agricultural University;Key Laboratory of Grassland Ecosystem of Ministry of Education/Sino-U.S.Centers for Grazing land Ecosystem Sustainability;Lanzhou 730070;China |
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| Abstract: | Effects of liquid culture medium types,concentrations and ventilation conditions on growth of phosphorus-dissolving Rhizobia SL01 strain were tested.The results indicated that the best liquid mediums for strains growth were LB and 1/2 LB,the proliferation rate of the strains was related to the type of mediums and nutrition condition as well.pH value of fermentation broth was greatly deviated from the initial acidity and alkalinity after being inoculated SL01.The density of SL01 in fermentation culture with ... |
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| Keywords: | Rhizobia culture medium ventilation condition |
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