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| 革胡子鲶出血性败血症病原菌的分离鉴定 | |
| 引用本文： | 王玉娟,王秀华,曲宪成,朱岩松,黄倢.革胡子鲶出血性败血症病原菌的分离鉴定[J].淡水渔业,2012,42(3):32-37. |
| 作者姓名： | 王玉娟 王秀华 曲宪成 朱岩松 黄倢 |
| 作者单位： | 1. 上海海洋大学水产与生命学院,上海201306;中国水产科学研究院黄海水产研究所,山东青岛266071 2. 中国水产科学研究院黄海水产研究所,山东青岛,266071 3. 上海海洋大学水产与生命学院,上海,201306 |
| 摘 要： | 从暴发性死亡的革胡子鲶(Clarias gariepinus)中分离出可疑病原菌株2010111403(简称1403),分别采用细菌全细胞脂肪酸鉴定系统、Biolog微生物自动鉴定系统及细菌16 S rDNA序列分析三种方法对其鉴定,结果表明菌株1403为嗜水气单胞菌(Aeromonas hydrophila)。分离菌株对健康革胡子鲶致病性测试表明其对革胡子鲶的半数致死剂量(LD50)为6.32×106菌落形成单位(CFU);实验感染革胡子鲶出现与自然发病相似症状,表明菌株1403是引起革胡子鲶发生暴发性死亡的主要致病病原。药敏试验表明,庆大霉素、四环素、阿奇霉素、氟哌酸、先锋霉素等9种抗生素对分离菌株有较强的抑制作用,但分离菌株对复方新诺明、克林霉素、罗红霉素等4种药物表现出耐药性。 |
| 关 键 词： | 革胡子鲶(Clarias gariepinus) 出血性败血症 嗜水气单胞菌(Aeromonas hydrophila) 分离鉴定 药敏测试 |
Isolation and identification of a bacterium associated with haemorrhagic septicaemia in walking catfish (Clarias gariepinus) |
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| WANG Yu-juan , WANG Xiu-hua , QU Xian-cheng , ZHU Yan-song , HUANG Jie.Isolation and identification of a bacterium associated with haemorrhagic septicaemia in walking catfish (Clarias gariepinus)[J].Freshwater Fisheries,2012,42(3):32-37. | |
| Authors: | WANG Yu-juan WANG Xiu-hua QU Xian-cheng ZHU Yan-song HUANG Jie |
| Institution: | Jie2(1.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China; 2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China) |
| Abstract: | An outbreak of haemorrhagic septicaemia that caused mass mortality was occurred in cultured Clarias gariepinus in Weifang,Shandong province in November 2010.In order to clarify the cause of the disease,streak plate method was used to isolate the bacteria from moribund fish.Three methods including gas chromatography of cellular fatty acids,biolog microbial identification system and 16 S rDNA sequence analysis were used to identify the isolated pathogen bacteria.LD50 value was used to evaluate the virulence of pathogen by challenge tests on C.gariepinus.The antimicrobial susceptibility testing was performed by disc diffusion method.A bacterial strain recovered from diseased C.gariepinus numbered 2010111403(hereinafter referred to as 1403)was dominant.Identification results by three methods all showed that bacterium 1403 was Aeromonas hydrophila.Challenge tests demonstrated bacterium 1403 was a virulent strain to C.gariepinus with LD50 approximate 6.32×106 CFU,with the similar symptoms as the naturally infected fish with haemorrhagic septicaemia.Antimicrobial susceptibility showed that bacterium 1403 were sensitive to 9 kinds of antibiotics such as gentamycin,minocycline,azithromycin,norfloxacin,cephalothin,etc,while it is resistant to 4 kinds of antibiotics including Sulfamethoxazolum-trimethoprimum,Clindamycin,Roxithromycin,ext. |
| Keywords: | Clarias gariepinus haemorrhagic septicaemia Aeromonas hydrophila isolation and identification Antimicrobial susceptibility test |
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