Application of random amplified polymorphic DNA to study genetic diversity in Paspalum scrobiculatum L. (Kodo millet, Poaceae) |
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| Authors: | H Kaburu M'Ribu Khidir W Hilu |
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| Institution: | (1) Department of Biology, Virginia Polytechnic Institute and State University, 24061 Blacksburg, Virginia, USA;(2) Present address: Department of Horticulture, Egerton University, P.O. Box 536, Njoro, Kenya;(3) Department of Biology, Virginia Polytechnic Institute and State University, 24061 Blacksburg, Virginia, USA |
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| Abstract: | Summary Genetic diversity and patterns of geographic variation among collections of Paspalum scrobiculatum (kodo millet) and P. polystachyum were studied using molecular markers generated through the random amplified polymorphic DNA (RAPD) method. A high level of polymorphism in RAPD markers was observed among the individual accessions, demonstrating the high genetic diversity of the crop. The markers obtained from the RAPD method were analyzed with the cluster analysis, principal coordinates and minimum spanning tree methods. Three major groups were resolved, one representing the African accessions, and two for the Indian accessions. The accessions of the north African kodo millet and P. polystachyum (considered conspecific with P. scrobiculatum) were quite distinct. The Australian kodo millet showed higher affinity to the African types. The study demonstrated that the RAPD technique can be applied to resolving degrees and patterns of genetic variation at the population and species levels, identifying cultivars, and defining gene pools of this crop. |
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| Keywords: | Paspalum kodo millet Poaceae grasses millets RAPD germplasm genetic diversity |
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