| 体外法优化肉鸡日粮非淀粉多糖酶 |
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| 引用本文： | 何科林,萨仁娜,高杰,李东卫,庄晓峰,张宏福.体外法优化肉鸡日粮非淀粉多糖酶[J].中国农业科学,2012,45(21):4457-4464. |
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| 作者姓名： | 何科林 萨仁娜 高杰 李东卫 庄晓峰 张宏福 |
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| 作者单位： | 1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室，北京 100193 |
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| 基金项目： | 农业部现代农业产业技术体系建设专项资金（CARS-42）、国家科技支撑计划项目（2012BDA39B01）、国家公益性行业科研专项经费（201003011-2） |
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| 摘 要： | 【目的】探讨非淀粉多糖酶对肉鸡玉米日粮和小麦日粮离体消化能的调控。【方法】首先采用单因素随机试验设计，测定非淀粉多糖酶与日粮离体消化能的剂量关系。5种非淀粉多糖（non-starch polysaccharides，NSP）酶分别为：木聚糖酶、β-葡聚糖酶、纤维素酶、果胶酶和β-甘露聚糖酶，在肉鸡玉米-豆粕和小麦-豆粕日粮中分别添加0、100、200、300、400、500、600、700、800和900 µg?g-1，分析NSP酶改善日粮离体消化能的作用效果。然后采用二次回归旋转正交组合试验设计，筛选两种日粮中5种NSP酶的最佳酶谱。离体消化能的测定采用胃蛋白酶-胰液素两步酶水解法。【结果】5种非淀粉多糖酶对离体消化能的调控效应显示规律性的“S”型曲线，木聚糖酶对玉米-豆粕、小麦-豆粕日粮的离体消化能提升程度较高，分别提高0.24和0.40 MJ?kg-1。在本试验条件下，玉米-豆粕日粮的最佳酶谱为：木聚糖酶34 836.4 U?kg-1、β-葡聚糖酶6 762.0 U?kg-1、纤维素酶1 159.2 U?kg-1、果胶酶872.6 U?kg-1和β-甘露聚糖酶24 535.9 U?kg-1；小麦-豆粕日粮的最佳酶谱为：木聚糖酶65 405.5 U?kg-1、β-葡聚糖酶10 131.5 U?kg-1、纤维素酶980.0 U?kg-1、果胶酶501.6 U?kg-1和β-甘露聚糖酶5 141.4 U?kg-1。【结论】肉鸡玉米-豆粕日粮、小麦-豆粕日粮中5种NSP酶联合使用能够改善日粮的离体消化能。
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| 关 键 词： | 非淀粉多糖酶 离体消化能 酶谱 肉仔鸡 体外法 |
| 收稿时间： | 2012-03-22 |
Study on Optimization of Non-Starch Polysaccharide Enzymes of Broiler Diets in Vitro |
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| HE Ke-Lin,SA Ren-Na,GAO Jie,LI Dong-Wei,ZHUANG Xiao-Feng,ZHANG Hong-Fu.Study on Optimization of Non-Starch Polysaccharide Enzymes of Broiler Diets in Vitro[J].Scientia Agricultura Sinica,2012,45(21):4457-4464. |
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| Authors: | HE Ke-Lin SA Ren-Na GAO Jie LI Dong-Wei ZHUANG Xiao-Feng ZHANG Hong-Fu |
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| Abstract: | 【Objective】The study was conducted to discuss the effects of non-starch polysaccharides (NSP) enzymes on the in vitro digestive energy (IVDE) of broiler diets of different contents of NSP.【Method】The first step was to investigate the dose-response of the NSP enzymes on the IVDE in diets using one-way randomized experimental design. Five NSP enzymes including xylanase, β-glucanase, cellulose, pectinase, and β-mannanase were added into corn-soybean diet and wheat-soybean diet, from 0 to 900 µg•g-1. The second step was to screen the optimum enzyme combinations using quadratic regress-orthogonal rotary design. The method of testing IVDE was a two-stage enzymic hydrolysis with pepsin-pancreatin in vitro.【Result】The IVDE of broiler diet regulating by NSP enzyme appeared S state diagram. The xylanase had more promotion effects than other NSP enzymes. It increased IVDE by 0.24 MJ•kg-1 and 0.40 MJ•kg-1 in corn-soybean diet and wheat-soybean diet, respectively. In corn-soybean diet, the optimum enzyme combinations were xylanase 34 836.4 U•kg-1, β-glucanase 6 762.0 U•kg-1, cellulose 1 159.2 U•kg-1, pectase 872.6 U•kg-1, and β-mannanase 24 535.9 U•kg-1. In wheat-soybean diet, the optimum enzyme combinations were xylanase 65 405.5 U•kg-1, β-glucanase 10 131.5 U•kg-1, cellulose 980.0 U•kg-1, pectase 501.6 U•kg-1, and β-mannanase 5 141.4 U•kg-1. 【Conclusion】The findings showed that using five kinds of NSP enzymes in corn-soybean and wheat-soybean diets of broiler could improve the in vitro digestive energy. |
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| Keywords: | NSP enzyme in vitro digestive energy enzyme combination broiler in vitro |
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