| 华南杂交稻亲本米质性状配合力的SSR标记鉴定 |
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| 引用本文： | 黄永相,郭建夫,王,慧,陈志强.华南杂交稻亲本米质性状配合力的SSR标记鉴定[J].南方农业学报,2012,43(12):1916-1921. |
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| 作者姓名： | 黄永相 郭建夫 王 慧 陈志强 |
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| 作者单位： | 广东海洋大学农学院,广东湛江524088华南农业大学国家植物航天育种工程技术研究中心,广州510642广东海洋大学农学院,广东湛江,524088华南农业大学国家植物航天育种工程技术研究中心,广州,510642 |
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| 基金项目： | 广东省重大科技专项项目（2007A020400003）；广东省现代农业产业技术体系建设专项资金项目（财教389号） |
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| 摘 要： | 目的]筛选与华南地区杂交稻亲本米质性状配合力连锁的分子标记,为华南地区杂交稻亲本米质改良和选配提供理论指导.方法]利用SSR标记技术,结合不完全双列杂交法,对华南地区18个杂交稻亲本配制的61个F1进行亲本米质性状相关性及配合力分子标记鉴定.结果]从糙米率、米粒长和米粒长宽比3个性状来看,不育系和恢复系对F1的相关系数相近;垩白粒率、垩白度、碱消值和直链淀粉含量等性状,恢复系与F1的相关系数则显著大于不育系,同时双亲间遗传距离与F1的相关系数也相对较高.经标记筛选获得了与这些性状极显著相关的17个亲本SSR分子标记,其中,直链淀粉含量6个,即RM5、RM250、RM274、RM549、RM336和RM427;碱消值5个,即RM236、RM18、RM274、RM336和RM427;垩白粒率两个,即RM5和RM276;糙米率、精米率、垩白度及胶稠度各1个,依次为RM274、RM246、RM276、RM549.结论]筛选的SSR标记可以直接用于华南杂交稻的米质预测及亲本配合力分子辅助育种改良.
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| 关 键 词： | 杂交稻 米质 配合力 SSR标记 相关分析 华南地区 |
Identification of SSR markers linked with quality grain traits and combining ability of elite hybrid rice parents in Southern China |
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| HUANG Yong-xiang,GUO Jian-fu,WANG Hui,CHEN Zhi-qiang.Identification of SSR markers linked with quality grain traits and combining ability of elite hybrid rice parents in Southern China[J].Journal of Southern Agriculture,2012,43(12):1916-1921. |
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| Authors: | HUANG Yong-xiang GUO Jian-fu WANG Hui CHEN Zhi-qiang |
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| Abstract: | 【Objective】The present experiment was conducted to screen for SSR markers linked with quality grain traits and combining ability of elite hybrid rice parental lines in Southern China area in order to provide basis for the selection and improvement of parental hybrid rice quality. 【Method】Utilizing the SSR marker technology combined with the incomplete diallel crosses method， the molecular markers of quality grain traits correlation and combining ability in 61 F1 traits of 18 hybrid rice parents in Southern China area were identified. 【Result】From analyzing the 3 traits of brown rice rate， rice grain length， and grain length-to-width ratio， the correlation coefficients between the F1 and male sterile lines and restorer lines were similar. The correlation coefficient between F1 and restoring lines was significantly greater than that of F1 and male sterile lines when the relevant chalky white grain rate， chalkiness degrees， alkali spreading values， and amylose contents were compared； at the same time， the correlation coefficient between the parents' genetic distance and F1 was relatively high. The 17 parent SSR molecular markers obtained were significantly related to these traits. Amongst the parent SSR molecular markers， there were 6 containing amylose contents， which were RM5， RM250， RM274， RM549， RM336， and RM427. Also from the parent SSR molecular markers， there were 5 containing alkali digestion value， which were RM236， RM18， RM274， RM336， and RM427. Two other parent SSR molecular markers contained chalky grain rate， which were RM5 and RM276. The remaining parent SSR markers each contain one of the following: brown rice rate， head rice rate， chalkiness degree， and gel consistency， which was respectively RM274， RM246， RM276， and RM549. 【Conclusion】SSR marker screening could be used directly for forecasting the hybrid rice grain quality in Southern China and for improving the combination ability of molecular assisted breeding. |
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