Development of a screening method for genetically modified soybean by plasmid-based quantitative competitive polymerase chain reaction |
| |
| Authors: | Shimizu Eri Kato Hisashi Nakagawa Yuki Kodama Takashi Futo Satoshi Minegishi Yasutaka Watanabe Takahiro Akiyama Hiroshi Teshima Reiko Furui Satoshi Hino Akihiro Kitta Kazumi |
| |
| Institution: | Fasmac Co., Ltd., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan. |
| |
| Abstract: | A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
