| “大通”牦牛Lfcin基因克隆及生物信息学分析(英文) |
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| 引用本文： | 裴杰,阎萍,姬国红,冯瑞林,梁春年,郭宪,曾玉峰,包鹏甲,褚敏.“大通”牦牛Lfcin基因克隆及生物信息学分析(英文)[J].Agricultural Science&Technology,2009(3). |
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| 作者姓名： | 裴杰 阎萍 姬国红 冯瑞林 梁春年 郭宪 曾玉峰 包鹏甲 褚敏 |
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| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所;甘肃农业大学草业学院; |
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| 基金项目： | Supported by Project of Basic Research Fund for National Non-profit Institute of China (BRF070105)~~ |
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| 摘 要： | Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.
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| 关 键 词： | 牦牛 外显子 奶牛 生物信息学分析 乳铁蛋白 序列比对 琼脂糖凝胶电泳 大通 基因编码区 序列分析 |
Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak |
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| PEI Jie,YAN Ping,JI Guo-hong,FENG Rui-lin,LIANG Chun-nian,GUO Xian,ZENG Yu-feng,BAO Peng-jia,CHU Min. Lanzhou Institute of Animal Science , Veterinary Pharmaceutical Science,Chinese Academy of Agricultural Science,Lanzhou ,. Gansu Agricultural University,College of Grass Science,Lanzhou.Cloning and Bioinformatics Analysis of Lfcin Gene of Datong Yak[J].农业科学与技术(英文版),2009(3). |
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| Authors: | PEI Jie YAN Ping JI Guo-hong FENG Rui-lin LIANG Chun-nian GUO Xian ZENG Yu-feng BAO Peng-jia CHU Min Lanzhou Institute of Animal Science Veterinary Pharmaceutical Science Chinese Academy of Agricultural Science Lanzhou Gansu Agricultural University College of Grass Science Lanzhou |
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| Institution: | PEI Jie1,YAN Ping1,JI Guo-hong2,FENG Rui-lin1,LIANG Chun-nian1,GUO Xian1,ZENG Yu-feng1,BAO Peng-jia1,CHU Min11. Lanzhou Institute of Animal Science , Veterinary Pharmaceutical Science,Chinese Academy of Agricultural Science,Lanzhou 730050,2. Gansu Agricultural University,College of Grass Science,Lanzhou 730070 |
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| Abstract: | Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, d... |
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| Keywords: | Yak Lfcin gene Clone Bioinformatics |
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