| 花椰菜下胚轴培养和高频率芽再生技术的研究 |
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| 引用本文： | 严慧玲,巩振辉,贾庆利.花椰菜下胚轴培养和高频率芽再生技术的研究[J].西北农林科技大学学报(社会科学版),2003,31(4):87-90. |
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| 作者姓名： | 严慧玲 巩振辉 贾庆利 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨陵,712100 |
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| 基金项目： | 科技部杨凌生物育种中心基金项目 ( 1999-18),陕西省自然科学基金 ( 2 0 0 1SM11),陕西省农业分子生物学重点实验室基金项目 |
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| 摘 要： | 以“春秋”花椰菜为试验材料 ,研究了影响下胚轴再生的若干因子。结果表明 ,6 d苗龄的下胚轴在附加 2～ 3m g/ L 6 - BA和 1.0 m g/ L NAA的 MS培养基上培养 ,芽分化率最高 ,达 93.33% ,平均每个外植体产芽5 .71个 ;Ag NO3对下胚轴的细胞生长有明显的促进作用 ;下胚轴的下端比上端产芽早 ,并且较易产生不定芽。
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| 关 键 词： | 花椰菜 下胚轴 离体培养 植株再生 |
| 文章编号： | 1671-9387(2003)04-0087-04 |
| 收稿时间： | 2002/9/20 0:00:00 |
| 修稿时间： | 2002年9月20日 |
High frequency of shoot regeneration from hypocotyls explants in Brassica oleracea var. botrytis |
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| YAN Hui ling,GONG Zhen hui,JIA Qing li.High frequency of shoot regeneration from hypocotyls explants in Brassica oleracea var. botrytis[J].Journal of Northwest Sci-Tech Univ of Agr and,2003,31(4):87-90. |
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| Authors: | YAN Hui ling GONG Zhen hui JIA Qing li |
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| Institution: | (College of Horticulture,Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | The hypocotyls explants from "Chunqiu" cauliflower were cultured and some factors affecting shoot regeneration were also studied.The results showed the highest frequency of shoot regeneration of hypocotyls explants from 6 d old in vitro grown seedling was 93.33% and No.of bud per explant with shoots was 5.71,using the MS media containing the hormone combinations of 6 BA 2-3 mg/L and NAA 0.5 mg/L.It was also found AgNO 3 (6 mg/L) was advantageous to the shoot formation;the lower segments of hypocotyls produced shoot earlier and easier than the upper segments. |
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| Keywords: | cauliflower hypocotyls in vitro plant regeneration |
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