Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples |
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| Authors: | Shim Won-Bo Yang Zheng-You Kim Ji-Young Choi Jin-Gil Je Jung-Hyun Kang Sung-Jo Kolosova Anna Yu Eremin Sergei A Chung Duck-Hwa |
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| Institution: | Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea. |
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| Abstract: | An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10 min to get results and one step of sample to perform the assay. The results of water samples spiked with 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtained by DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screening of atrazine in various water samples. |
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