| 火龙果组培增殖快繁技术研究 |
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| 引用本文： | 刘洪章,刘艳军,苗博瑛.火龙果组培增殖快繁技术研究[J].天津农业科学,2012,18(5):32-34. |
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| 作者姓名： | 刘洪章 刘艳军 苗博瑛 |
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| 作者单位： | 1. 天津市林业果树研究所,天津,300192
2. 天津农学院园艺系,天津,300384 |
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| 摘 要： | 为了建立火龙果的组培快繁体系,以火龙果的茎段为外植体,消毒后接种到含有不同浓度的6-BA和IAA的MS培养基上,从中筛选出增殖系数最高的培养基配方;将增殖出的火龙果芽接种到含有不同浓度的6-BA和IAA的MS培养基上,从中筛选出最好的伸长培养基配方;将生长到4cm的火龙果幼苗接种到含有不同生长素的生根培养基上,从中筛选出生根效果最好的培养基配方。试验结果显示,最好的增殖培养基配方是:MS+6-BA3.0mg·L-1+IAA0.5mg·L-1;最好的伸长培养基配方是:MS+6-BA1.0mg·L-1+IAA1.0mg·L-1;最好的生根培养基是:1/2MS+IAA1.0mg·L-1。
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| 关 键 词： | 火龙果 组织培养 快繁 培养基 |
Tissue Culture and Rapid Propagation of Hylocereus undatus |
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| LIU Hong-zhang
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LIU Yan-jun
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MIAO Bo-ying.Tissue Culture and Rapid Propagation of Hylocereus undatus[J].Tianjin Agricultural Sciences,2012,18(5):32-34. |
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| Authors: | LIU Hong-zhang LIU Yan-jun MIAO Bo-ying |
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| Institution: | 1 (1.Tianjin Research Institute of Forestry and Pomology,Tianjin 300192,China; 2.Horticultral Department, Tianjin Agricultural University,Tianjin 300384,China) |
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| Abstract: | In order to establish the system of Hylocereus undatus tissue culture and rapid propagation, the stem sections of Hylocereus undatus were disinfected . The buds of Hylocereus undatus were regenerated from the disinfect stem sections on MS medium containing 6-BA and IAA of different concentration. The buds of regeneration were cut down and inoculated on MS medium containing 6-BA and IAA of different concentration for elongation. When the buds for elongation were 4 cm high, they were cut down and inoculated on MS medium containing auxin of different concentration for rooting. The experimental results suggested that the optima1 media for buds regeneration were:MS +6 -BA3.0 mg·L -1 +IAA0.5 mg·L -1;the optima1 media for elongation were:MS +6 -BA1.0 mg·L-1+IAA1.0 mg·L-1; the optima1 media for rooting were1/2MS +IAA1.0 mg·L-1. |
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| Keywords: | Hylocereus undatus tissue culture rapid propagation medium |
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