| 两种膜荚黄芪DNA提取方法的优化与比较 |
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| 引用本文： | 赵成萍,雷万钧,刘亚令,梁建萍.两种膜荚黄芪DNA提取方法的优化与比较[J].天津农业科学,2012,18(3):5-8. |
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| 作者姓名： | 赵成萍 雷万钧 刘亚令 梁建萍 |
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| 作者单位： | 山西农业大学生命科学学院,山西太谷,030801 |
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| 基金项目： | 山西省科技攻关项目(20110313002-2);博士启动基金(XB2008022) |
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| 摘 要： | 以膜荚黄芪的嫩叶片为材料,对CTAB法和高盐低pH法进行了改良,并用此改良法提取总DNA,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。结果表明,无论从提取率还是纯度上,优化的高盐低pH法都优于CTAB法,但高盐低pH法提取的DNA不适合长期保存。
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| 关 键 词： | 膜荚黄芪 DNA提取 CTAB法 高盐低pH法 |
Optimization and Comparison of Two Total DNA Extraction Methods from Astragalus membranaceus |
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| ZHAO Cheng-ping
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LEI Wan-jun
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LIU Ya-ling
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LIANG Jian-ping.Optimization and Comparison of Two Total DNA Extraction Methods from Astragalus membranaceus[J].Tianjin Agricultural Sciences,2012,18(3):5-8. |
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| Authors: | ZHAO Cheng-ping LEI Wan-jun LIU Ya-ling LIANG Jian-ping |
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| Institution: | ( College of Life Science, Shanxi Agricultural University, Taigu ,Shanxi 030801,China) |
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| Abstract: | In this paper, the CTAB method and high-salt low-pH method was modified and improved to extract total DNA using the young leaves of Astragalus membranaceus. Then the DNA quality was detected by UV spectrophotometry and agarose gel electrophoresis. The results showed that the optimization of high-salt low-pH method was superior to CTAB method in terms of extraction rate or purity, but the extracted DNA was not suitable for long-term preservation. |
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| Keywords: | Astragalus membranaceus DNA extraction CTAB method high-salt low-pH method |
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