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| 对乙酰氨基酚对大鼠原代肝细胞的损伤机制 | |
| 引用本文： | 鞠 雷,王宝杰,马玉忠.对乙酰氨基酚对大鼠原代肝细胞的损伤机制[J].中国农学通报,2008,24(11):24-26. |
| 作者姓名： | 鞠 雷 王宝杰 马玉忠 |
| 作者单位： | 1. 河北农业大学动物科技学院,保定,071001;山东畜牧兽医职业学院,潍坊,261061 2. 山东畜牧兽医职业学院,潍坊,261061 3. 河北农业大学动物科技学院,保定,071001 |
| 基金项目： | 河北农业大学校科研和教改项目 |
| 摘 要： | 胶原酶两步灌流法获取SD大鼠的原代肝细胞，并用不同剂量的对乙酰氨基酚处理，MTT法测定肝细胞的存活率，分光光度法测定培养上清液中乳酸脱氢酶（LDH）的含量。Western blotting法测定大鼠肝细胞中细胞色素P450酶2E1(CYP2E1)的表达水平。结果表明，对乙酰氨基酚可导致肝细胞存活率降低，LDH含量显著升高，CYP2E1表达增加。说明达到一定的剂量时，对乙酰氨基酚可引起肝细胞的损伤。 |
| 关 键 词： | 大鼠 原代肝细胞 对乙酰氨基酚 损伤机制 |
| 收稿时间： | 2008-07-28 |
| 修稿时间： | 2008-08-27 |
Mechanism of Rat Primary Cultured Hepatocyte Injury by Acetaminophen |
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| Ju Lei,Wang Baojie,Ma Yuzhong.Mechanism of Rat Primary Cultured Hepatocyte Injury by Acetaminophen[J].Chinese Agricultural Science Bulletin,2008,24(11):24-26. | |
| Authors: | Ju Lei Wang Baojie Ma Yuzhong |
| Abstract: | Rat hepatocytes were obtained from SD rats by two step collagenase perfusion, and treated with different concentration of acetaminophen.The hepatocyte viability was measured by MTT, the activities of lactate dehydrogenase(LDH) was analyzed by spectrophotometer, and the expression of CYP 2E1 were measured by western blotting.With induction of acetaminophen, the hepatocyte viability decreased, the activity of LDH increased, the expression of CYP 2E1 increased.It demonstrated that acetaminophen could lead to hepatocyte injury when acetaminophen reached to higher dose. |
| Keywords: | rat primary cultured hepatocytes acetaminophen mechanism |
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