| 高效液相色谱法同时测定液态乳中的2种紫外吸收剂和2种抗氧化剂 |
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| 引用本文： | 张大原,李挥,张兰天,艾连峰.高效液相色谱法同时测定液态乳中的2种紫外吸收剂和2种抗氧化剂[J].乳业科学与技术,2016,39(2):17-20. |
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| 作者姓名： | 张大原 李挥 张兰天 艾连峰 |
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| 作者单位： | 河北省食品检验研究院,河北省食品安全重点实验室,河北石家庄 050091;河北衡水中学,河北衡水 053000；河北省食品检验研究院,河北省食品安全重点实验室,河北石家庄 050091；河北出入境检验检疫局,检验检疫技术中心,河北石家庄 050051 |
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| 基金项目： | 河北省科技计划项目(15275505D) |
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| 摘 要： | 建立液态乳中2种紫外吸收剂(UV-326和UV-1577)和2种抗氧化剂(AO-1010、AO-2246)的高效液相色谱测定方法.前处理采用乙腈提取液态乳中目标化合物,样品用正己烷提取;检测经Agilent HC-C8反相色谱柱,以乙腈-水作为流动相,梯度洗脱程序进行分离,280 nm波长下检测.结果表明:分析物在0.05～50 μg/mL质量浓度范围内线性关系良好,线性相关系数＞0.999 5,方法检出限为0.02～0.5 mg/kg.方法在3个水平的加标回收率为69.08％～115.87％,相对标准偏差为0.43％～9.94％.该方法操作简单、灵敏度高、重现性好,适用于液态乳中紫外吸收剂和抗氧化剂的同时测定.
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| 关 键 词： | 高效液相色谱 紫外吸收剂 抗氧化剂 液态乳 |
Simultaneous Determination of 2 Ultraviolet Absorbers and 2 Antioxidants in Milk by High Performance Liquid Chromatography |
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| ZHANG Dayuan,LI Hui,ZHANG Lantian,AI Lianfeng.Simultaneous Determination of 2 Ultraviolet Absorbers and 2 Antioxidants in Milk by High Performance Liquid Chromatography[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2016,39(2):17-20. |
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| Authors: | ZHANG Dayuan LI Hui ZHANG Lantian AI Lianfeng |
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| Institution: | (1.Hebei Key Laboratory of Food Safety, Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China;
2.Hebei Hengshui High School, Hengshui 053000, China; 3.Hebei Entry-Exit Inspection and Quarantine Technical Center,
Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China) |
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| Abstract: | A method was developed for the simultaneous determination of 2 ultraviolet absorbers UV-326, UV-1577 and
2 antioxidants AO-1010 and AO-2246 in milk by high performance liquid chromatography. Samples were extracted with
acetonitrile. The effective separation of the target analytes was accomplished on Agilent HC-C8 (150 mm × 4.6 mm, 5.0 μm)
column with gradient elution procedure using a mixture of water and acetonitrile as the mobile phase. External calibration
was used for quantitative determination at detection wavelength of 280 nm. The linearity of 4 analytes were satisfactory
with a correlation coefficient of > 0.9995 at concentrations ranging from 0.05 to 50 μg/mL. The limits of detection of the
method were 0.02?0.5 mg/kg, and the recoveries at different spiked levels and precision (RSD) were 69.08%?115.87% and
0.43%?9.94%, respectively. The method is simple, sensitive, reproducible and suitable for the simultaneous determination of
2 ultraviolet absorbers and 2 antioxidants in milk. |
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| Keywords: | high performance liquid chromatography (HPLC) ultraviolet absorber antioxidants milk |
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