A method of experimental infection of kuruma shrimp larvae, Penaeus japonicus Bate, with baculoviral mid-gut gland necrosis (BMN) virus |
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| Authors: | K MOMOYAMA T SANO |
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| Institution: | Yamaguchi Prefectural Naikai Fisheries Experimental Station, Yamaguchi, Japan;Laboratory of Fish Pathology, Tokyo University of Fisheries, Tokyo, Japan |
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| Abstract: | Abstract. Infectivity experiments were undertaken by water-borne inoculation of kuruma shrimp, Penaeus japonicus Bate, larvae with BMN virus. Mysis stage larvae were inoculated with the virus by exposure for 2h in sea water containing a homogenized and filtered preparation (450nm) of naturally BMN virus-infected shrimp stored for about 7 weeks at ?80°C. Inoculated shrimp stocked in rearing jars were examined to determine whether nuclear hypertrophy of the mid-gut gland epithelial cells characteristic for BMN virus infection could be observed in fresh squash preparations under dark field illumination equipped with a wet-type condenser. Four days post-inoculation at 25–30°C incubation temperature were considered to be satisfactory for the experimental trial. |
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