REP-PCR Analysis of Pasteurella multocida Isolates from Wild and Domestic Animals in India |
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| Authors: | M K Saxena V P Singh A A Kumar P Chaudhuri Vijendra Pal Singh S B Shivachandra A Biswas B Sharma |
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| Institution: | (1) Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, U.P., India;(2) Division of Biochemistry and Food Science, Indian Veterinary Research Institute, Izatnagar, U.P., India |
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| Abstract: | Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was used to characterize 67 field isolates of Pasteurella multocida originating from different animal species and geographical regions of India. REP-PCR was found to be rapid and reproducible
(three repeats were done). These isolates yielded different 23 profiles which were clustered into eight groups. The discrimination
index was moderate (D value 0.83). Somatic and antigenic typing of the isolates did not reveal any correlation with REP-PCR profiles. There was
no host-specific, type-specific, region-specific or pathenogenicity-specific pattern. The REP profiles of isolates obtained
from wild animals were similar to those obtained from domestic animals. Two common bands were present in all the isolates
irrespective of somatic or antigenic types. The results were not comparable with earlier findings, which had shown high discrimination
index and correlation with disease presentation.
Saxena, M.K., Singh, V.P., Kumar, A.A., Chaudhuri, P., Singh, V.P., Shivachandra, S.B., Biswas, A. and Sharma, B., 2006. REP–PCR
analysis of Pasteurella multocida isolates from wild and domestic animals in India. Veterinary Research Communications, 30(8), 851–861 |
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| Keywords: | REP-PCR Pasteurella multocida differentiation coefficient dendogram |
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