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| 空间诱变水稻H4成熟胚再生体系的建立 | |
| 引用本文： | 周丹华,孙大元,王慧,陈志强,王国宝,余国新.空间诱变水稻H4成熟胚再生体系的建立[J].广东农业科学,2013,40(7):1-3. |
| 作者姓名： | 周丹华 孙大元 王慧 陈志强 王国宝 余国新 |
| 作者单位： | 1. 湖南农业大学资源环境学院,湖南长沙410000;环境保护部华南环境科学研究所,广东广州510655 2. 环境保护部华南环境科学研究所,广东广州,510655 3. 华南农业大学农学院,广东广州,510642 |
| 基金项目： | 国家环保公益性行业科研专项 |
| 摘 要： | 通过室内盆栽模拟方法,研究不同基因型水稻品种(天优428、天优998)对酞酸酯胁迫的响应,为酞酸酯污染农田的水稻栽培提供依据。结果表明,酞酸酯对两种水稻生长前期的株高有增效作用,在开花期和成熟期对两个水稻品种株高的影响略有不同,天优998的株高随着PAEs浓度的增高有所增加;酞酸酯处理后水稻的分蘖数、有效穗数减少,产量显著降低。同时酞酸酯处理对水稻的光合速率、蒸腾速率、MDA含量和可溶性糖含量等有一定的影响。综合所得结果,天优998较适于在酞酸酯污染农田中种植。 |
| 关 键 词： | 酞酸酯 水稻 胁迫 生长 |
Study on the regeneration system from mature embryo of the indica rice H4 |
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| YANG Zi-jiang , RAO Gang-shun , XIAO Li-zhong , LI Rong-fei , CAI Xin-de.Study on the regeneration system from mature embryo of the indica rice H4[J].Guangdong Agricultural Sciences,2013,40(7):1-3. | |
| Authors: | YANG Zi-jiang RAO Gang-shun XIAO Li-zhong LI Rong-fei CAI Xin-de |
| Institution: | 1.College of Resources and Environment,Hunan Agricultural University,Changsha 410000,China;2.Ministry of Environmental Protection,South China Institute of Environmental Sciences,Guangzhou 510655,China;3.College of Agriculture,South China Agricultural University,Guangzhou 510655,China) |
| Abstract: | To establish a suitable regeneration system of space-induced indica rice, H4, a series of experimental programs were carried out. The results showed that When NMB was used as the basic medium, 2.5 mg/L 2,4-D, 1.0 mg/L NAA and 0.5 mg/L 6-BA were added, the callus induction frequency of H4 was 91.63%; ABA showed a significant result on the growth and regeneration of callus; the two -step culture method exhibited positive effects on enhancing plant regeneration ability. The regeneration technology for high frequency of H4 mature embryos was established preliminarily. |
| Keywords: | space mutation mature embryo tissue culture |
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