| 黄花白芨组培快繁技术 |
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| 引用本文： | 朱玉球,王雪根.黄花白芨组培快繁技术[J].浙江农林大学学报,1999,16(2):164-169. |
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| 作者姓名： | 朱玉球 王雪根 |
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| 作者单位： | 1.浙江林学院 资源与环境系, 浙江临安 311300;2.浙江省临安市锦城林业站, 浙江 临安 311300 |
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| 摘 要： | 用不同的基本培养基、不同的植物生长调节剂及其配比、不同的原球茎切割方式系统地研究了黄花白芨(Bleti lla ochracea)组织培养技术。试验结果表明:1/2 MS +1.0 mgL-1 BA +0.1 mgL-1 NAA 培养基有利于原球茎增殖, 培养60 d 增殖到4.21 倍;Kyoto +1.0 mgL-1 BA +0.1 mgL-1 NAA 的培养基有利于诱导原球茎增殖、分化及幼苗的生长, 培养60 d 分化形成的芽数为接种原球茎数的4.79 倍;Kyoto +2.0 mgL-1 BA +0.1 mgL-1 NAA 的培养基对球茎切块诱导芽的效果最好。在继代培养中, 应采用纵切法切割球茎或用自然掰开法来分割原球茎。同时, 对进一步的研究提出了建议。表5 参4
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| 关 键 词： | 黄花白芨 组织培养 原球茎 繁殖 分化 |
| 收稿时间： | 1998-09-28 |
Fast propagation techniques of Beltilla ochracea by means of tissue cultures |
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| ZHU Yu-qiu,WANG Xue-gen.Fast propagation techniques of Beltilla ochracea by means of tissue cultures[J].Journal of Zhejiang A&F University,1999,16(2):164-169. |
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| Authors: | ZHU Yu-qiu WANG Xue-gen |
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| Institution: | 1.Department of Resources and Environment , Zhejiang Forestry College , Lin’an 311300, China;2.Jincheng Forest Station of Lin’an City , Lin’an 311300, China |
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| Abstract: | A study of techniques for tissue cultures of Bletilla ochracea was made in terms of basic culture media , hormones and their proportions and the cutting of original bulbs .Its showed that the medium compose of 1/2MS +1.0 mgL-1BA +0.1 mgL-1NAA was favorable to the proliferation of the original bulb , with 4.21 times as many as thd original proliferated in 60 days ;the medium consisting of Kyoto +1.0 mgL-1BA +0.1mgL-1NAA was favorable to the induction of the original bulb to proliferate and differentiate and the growth of seedlings , with 4.79 times as many as the number of buds of the original bulb differentiated and formed within 60 days ;and the medium with Kyoto +2.0 mgL-1BA +0.1 mgL-1NAA involved had a good effect on the bud induction of pieces cut from the original bulb .In the culture of subsequent generations , vertical cutting of the bulb or natural separation of the original bulb should be used .Some suggestions for further studies are put forward meanwhile . |
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