| 扶芳藤不定芽再生体系的建立 |
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| 引用本文： | 王茂良,赵梁军,冯慧,王建红.扶芳藤不定芽再生体系的建立[J].北京农学院学报,2010,25(1):60-64. |
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| 作者姓名： | 王茂良 赵梁军 冯慧 王建红 |
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| 作者单位： | 北京市园林科学研究所,北京,100102;中国农业大学观赏园艺与园林系,北京,100193;中国农业大学观赏园艺与园林系,北京,100193;北京市园林科学研究所,北京,100102 |
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| 基金项目： | 北京市科技计划项目(H020620110130) |
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| 摘 要： | 以扶芳藤无芽茎段和下胚轴为外植体,建立了稳定高效的不定芽再生体系。MS＋6-BA 1.5 mg/L＋IBA0.1 mg/L对诱导无芽茎段产生不定芽最为有利,再生率为82.6%。以下胚轴为外植体时,预培养50 d更有利于不定芽的诱导,中浓度的6-BA（1.3 mg/L和1.5 mg/L）对直接分化产生不定芽有利,培养基MS＋6-BA1.5 mg/L＋NAA0.1 mg/L上不定芽再生率最高,达到95.7%。以绵白糖和蔗糖为碳源均能达到较好的再生效果（80%以上）,以葡萄糖为碳源时再生率虽然高达100%,但玻璃化严重。生根培养基以1/2 MS＋IAA（0.7 mg/L）最好,生根率高达92.9%,经驯化后移栽成活率在90%以上。
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| 关 键 词： | 扶芳藤 无芽茎段 下胚轴 不定芽再生 再生体系 |
Establishment of adventitious bud regenerating system from in vitro Euonymus fortunei |
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| WANG Mao-liang,ZHAO Liang-jun,FENG Hui,WANG Jian-hong.Establishment of adventitious bud regenerating system from in vitro Euonymus fortunei[J].Journal of Beijing Agricultural College,2010,25(1):60-64. |
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| Authors: | WANG Mao-liang ZHAO Liang-jun FENG Hui WANG Jian-hong |
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| Institution: | 1.Beijing Institute of Landscape Architecture;Beijing 100102;China;2.Department of Ornamental Horticulture and Landscape Architecture;China Agriculture University;Beijing 100193;China |
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| Abstract: | An efficient adventitious bud regeneration system of E.fortuneiwas established when inter-node and hypocotyls segments were used as explants.The optimum medium for induction of adventitious buds from inter-node of E.fortunei was MS+6-BA 1.5 mg/L +IBA 0.1 mg/L,and the regeneration rate of adventitious buds reached 82.6%.For hypocotyls explants,50 d pre-culture and 6-BA at 1.3 mg/L to 1.5 mg/L were efficient to adventitious buds induction.The perfect medium for buds regeneration from hypocotyls segments was M... |
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| Keywords: | Euonymus fortunei inter-node stem-segment hypocotyls segment adventitious buds regeneration regenerating system |
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