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| 盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响 | |
| 引用本文： | 杨晓红,陈晓阳,李守菊,隗晓丹,王颖,周伟.盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响[J].北京林业大学学报,2009,31(4). |
| 作者姓名： | 杨晓红 陈晓阳 李守菊 隗晓丹 王颖 周伟 |
| 作者单位： | 1. 北京林业大学林木花卉遗传育种教育部重点实验室;北京农学院园林系 2. 北京林业大学林木花卉遗传育种教育部重点实验室;华南农业大学林学院 3. 北京农学院园林系 |
| 基金项目： | 国家高技术研究发展计划(863计划)，北京市科委资助项目 |
| 摘 要： | 为了探讨盐胁迫下外源基因对植物渗透调节的影响,以同时培育的转入果聚糖蔗糖转移酶(SacB)基因、转甜菜碱醛脱氢酶(BADH)基因及未转基因的美丽胡枝子盆栽苗为材料,研究不同浓度(0、0.5%、1.0%、2.0%)NaCl处理对3种试验材料的耐盐性及盐胁迫下的脯氨酸、甜菜碱、可溶性糖、丙二醛含量、过氧化物歧化酶活性的影响。结果表明:在未进行盐胁迫时,3种试材的这几项指标含量没有明显差异,但随着盐胁迫强度的增加,两种转基因的美丽胡枝子在积累脯氨酸、可溶性糖能力方面明显强于非转基因植株,转入BADH基因的美丽胡枝子在积累甜菜碱上要强于非转基因植株及转入SacB基因的植株;尽管在盐胁迫强度增大的情况下,3种植株的过氧化物歧化酶活性增强了,但两种转基因植株的过氧化物歧化酶活性并没有明显大于非转基因植株;两种转基因植株可明显抑制丙二醛在植物体内的快速积累。 |
| 关 键 词： | 转SacB基因植株 转BADH基因植株 美丽胡枝子 盐胁迫 逆境生理指标 |
Effects of salt stress on some stress physiological indexes of SacB-transgenic & BADH-transgenic Lespedeza formosa plants |
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| YANG Xiao-hong,CHEN Xiao-yang,LI Shou-ju,WEI Xiao-dan,WANG Ying,ZHOU Wei.Effects of salt stress on some stress physiological indexes of SacB-transgenic & BADH-transgenic Lespedeza formosa plants[J].Journal of Beijing Forestry University,2009,31(4). | |
| Authors: | YANG Xiao-hong CHEN Xiao-yang LI Shou-ju WEI Xiao-dan WANG Ying ZHOU Wei |
| Institution: | YANG Xiao-hong~(1,2),CHEN Xiao-yang~(1,3),LI Shou-ju~2,WEI Xiao-dan~2,WANG Ying~2,ZHOU Wei~2.1 Key Laboratory for Genetics , Breeding in Forest Trees , Ornamental Plants of Ministry of Education,Beijing Forestry University,100083,P.R.China,2 L,scape Department,Beijing University of Agriculture,102206,3 College of Forestry,South China Agricultural University,Guangzhou,510642,P.R.China. |
| Abstract: | SacB-transgenic,BADH-transgenic and non-transgenic Lespedeza formosa potted plants were selected to explore the effects of exogenous gene on osmotic regulation of plants under salt stress.All plants were cultured under same conditions and were treated with NaCl solutions.Salt stress tolerance of 3 types of plant materials was compared.Several physiological indexes,including proline,betaine,malonalohyde(MDA),superoxide dismutase(SOD) and soluble sugar were assayed when three L.formosa material types were tre... |
| Keywords: | SacB-transgenic plants BADH-transgenic plants Lespedeza formosa salt stress stress physiological indexes |
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