| 矮牵牛花药培养胚状体诱导和植株再生 |
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| 引用本文： | 鲁娇娇,张青,王平,侯超.矮牵牛花药培养胚状体诱导和植株再生[J].安徽农业科学,2013,41(5):1907+1960. |
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| 作者姓名： | 鲁娇娇 张青 王平 侯超 |
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| 作者单位： | 辽宁省农业科学院,辽宁沈阳,110161;瑞克斯旺中国种子有限公司,江苏宿迁,223800 |
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| 摘 要： | 目的]利用矮牵牛的花药培养诱导胚状体和再生植株。方法]以7个品种矮牵牛的花药为材料,研究其胚状体及其再生植株的诱导方法。结果]基因型和培养基成分对胚状体的诱导有重要影响;椰乳和活性炭对胚状体的发生及发育有促进作用;向改良MS培养基中添加0.01%活性炭可促进植株再生;最佳生根培养基为1/2MS+IBA 0.2 mg/L。结论]该研究为矮牵牛的大规模工业化生产奠定了基础。
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| 关 键 词： | 矮牵牛 花药 胚状体 植株再生 |
Embryoid Induction and Plant Regeneration of Petunia hybrida vilm by Its Anther Culture |
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| Institution: | LU Jiao-jiao et al(Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161) |
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| Abstract: | Objective] The paper was to induce the embryoid and regenerated plants of Petunia hybrida vilm by its anther culture.Method] The induction methods of embryoid and regenerated plants were studied by using anther of 7 species of P.hybrida as materials.Result] Genotype and medium composition had important effects on the induction of embryoid;coconut milk and activated carbon could promote the induction and development of embryoid;the modified MS medium supplemented with 0.01% activated charcoal could promote the regeneration of plants;the best rooting medium was 1/2MS+IBA 0.2 mg/L.Conclusion] The study laid the foundation for the large-scale industrialization production of P.hybrida. |
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| Keywords: | Petunia hybrida vilm Anther Embryoid Plant regeneration |
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