| 甜樱桃茎尖培养及PNRSV的RT-PCR检测 |
| |
| 引用本文： | 代红艳,张志宏,吴禄平,侯义龙,吕德国.甜樱桃茎尖培养及PNRSV的RT-PCR检测[J].园艺学报,2003,30(1):87-91. |
| |
| 作者姓名： | 代红艳 张志宏 吴禄平 侯义龙 吕德国 |
| |
| 作者单位： | (沈阳农业大学园艺学院, 沈阳110161 ;大连大学生物工程学院, 大连116622) |
| |
| 摘 要： | 研究了茎尖大小、接种方式、培养基成分、试材基因型对甜樱桃品种茎尖培养的影响。1 年生成熟枝条上茎尖成苗率为8. 3 %～23. 7 % , 嫩梢上的茎尖成苗率为27. 3 %～37. 5 %。利用RT-PCR 技术对部分甜樱桃试管苗进行了早期病毒鉴定, 筛选出一些不带李坏死环斑病毒(PNRSV) 的甜樱桃试管苗,并证明甜樱桃试管苗微茎尖培养不能有效脱除PNRSV。
|
| 关 键 词： | 甜樱桃 茎尖培养 病毒 RT-PCR |
| 文章编号： | 0513-353X(2003)01-0087-03 |
| 收稿时间： | 2002-3-12 |
| 修稿时间： | 2002年3月12日 |
In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSV by RT-PCR |
| |
| Dai Hongyan,Zhang Zhihong,Wu Luping,Hou Yilong,LüDeguo.In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSV by RT-PCR[J].Acta Horticulturae Sinica,2003,30(1):87-91. |
| |
| Authors: | Dai Hongyan Zhang Zhihong Wu Luping Hou Yilong LüDeguo |
| |
| Institution: | Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang 110001,China |
| |
| Abstract: | The effects of size of inoculated shoot tip, method of inoculation, medium and genotype on shoot tip culture of sweet cherry ( Prunus avium L.) cultivars were studied. The rate of shoots forming from tips of one year old twigs was low, 8.3% to 23.7%, while the rate of shoots forming from tips of actively growing shoots was higher, 27.3% to 37.5%. Prunus necrotic ringspot virus (PNRSV) was detected early in some of sweet cherry plantlets in vitro with RT PCR technique. Plants free of PNRSV were found. It was proved that PNRSV could not be eliminated effectively from sweet cherry plantlets in vitro by culture of minor shoot tip. |
| |
| Keywords: | Sweet cherry Shoot tip culture Virus RT PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
