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| 苏太仔猪FUT1基因M307位点多态性与F18大肠杆菌抗病相关性的体外鉴定 | |
| 引用本文： | 吴圣龙,原志伟,鞠慧萍,黄雪根,华金弟,沈家林,周冠月,王建业,谢恺舟,陈国宏,朱国强.苏太仔猪FUT1基因M307位点多态性与F18大肠杆菌抗病相关性的体外鉴定[J].中国预防兽医学报,2007,29(10):783-787. |
| 作者姓名： | 吴圣龙 原志伟 鞠慧萍 黄雪根 华金弟 沈家林 周冠月 王建业 谢恺舟 陈国宏 朱国强 |
| 作者单位： | 1. 扬州大学,兽医学院,江苏,扬州,225009;扬州大学,动物科学与技术学院,江苏,扬州,225009 2. 扬州大学,兽医学院,江苏,扬州,225009 3. 扬州大学,动物科学与技术学院,江苏,扬州,225009 4. 苏州市苏太猪育种中心,江苏,苏州,215000 |
| 基金项目： | 江苏省六大人才高峰基金;江苏省高技术(农业)项目 |
| 摘 要： | 采用PCR-RFLP方法检测了江苏苏太断奶仔猪FUT1基因M307位点等位基因多态性分布,在所检的49头仔猪中,GG基因型个体16头,AG基因型19头,AA基因型14头。在此基础上,制备上述不同基因型个体仔猪小肠上皮细胞,分别与表达F18ab菌毛的野生型大肠杆菌、表达F18ac菌毛含fed操纵子全基因的重组大肠杆菌和V型系统表面分泌表达F18abFedF亚单位的重组大肠杆菌进行体外黏附试验和黏附抑制试验。研究结果表明:FUT1基因M307位点中GG型和AG型仔猪小肠上皮细胞均能黏附上述3种大肠杆菌,而AA型个体小肠上皮细胞则不能黏附。将上述3种大肠杆菌分别与抗F18ab菌毛高免血清、F18ac菌毛高免血清及抗F18abFedF亚单位单因子血清作用后,则失去黏附仔猪肠上皮细胞能力。上述结果对苏太猪从体外试验上证明了FUT1基因M307位点多态性与断奶仔猪腹泻和水肿病存在着直接的相关性。 |
| 关 键 词： | 仔猪 F18大肠杆菌 受体 FUT1基因 基因多态性 |
| 文章编号： | 1008-0589（2007）10-0783-05 |
| 修稿时间： | 2007-02-18 |
Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F18 fimbrial Escherichia coli in vitro |
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| WU Sheng-long,YUAN Zhi-wei,JU Hui-ping,HUANG Xue-gen,HUA Jin-di,SHEN Jia-lin,ZHOU Guan-yue,WANG Jian-ye,XIE Kai-zhou,CHEN Guo-hong,ZHU Guo-qiang.Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F18 fimbrial Escherichia coli in vitro[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(10):783-787. | |
| Authors: | WU Sheng-long YUAN Zhi-wei JU Hui-ping HUANG Xue-gen HUA Jin-di SHEN Jia-lin ZHOU Guan-yue WANG Jian-ye XIE Kai-zhou CHEN Guo-hong ZHU Guo-qiang |
| Institution: | 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 3. Center of Sutai Breeding, Suzhou 215000, China |
| Abstract: | The polymorphisms of FUT1 gene M307 locus in 49 post-weaning Sutai breed piglets were screened by PCR-RFLP,and three genotype were identified as FUT1 M307GG(16),M307AG(19) and M307AA(14).Small intestinal epithelium cells of different genotypes were selected to test the adhesion capability of the wild type E.coli expressing F18ab fimbriae,the recombinant Escherichia coli expressing F18ac fimbriae or the recombinant Escherichia coli secreting and surface-displaying the FedF subunit of F18ab fimbriae,respectively.The results showed that all of wild type and recombinant Escherichia coli were able to adhere to the post-weaning small intestinal epithelium cells of both M307GG and M307AG genotypes.The adhesion capability could be inhibited after incubation bacterium with the corresponding anti-serium.There appeared to be a direct relationship between polymorphisms of FUT1 gene M307 locus in post-weaning Sutai breed piglets and the resistance to pathogenic F18 fimbrial E.coli,which could be determined by adhesion and adhesion inhibition assays in vitro.The M307GG and M307AG genotypes are related to the susceptibility of the PWD and ED,while M307AA related to the resistance. |
| Keywords: | piglet Escherichia coli F18 receptor FUT1 gene polymorphism |
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