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液体发酵平菇菌株的诱变选育
引用本文:王淑芳,卜庆梅,刘泽隆,杨立红.液体发酵平菇菌株的诱变选育[J].安徽农业科学,2006,34(9):1858-1859.
作者姓名:王淑芳  卜庆梅  刘泽隆  杨立红
作者单位:烟台师范学院生命科学学院,山东,烟台,264025;烟台师范学院生命科学学院,山东,烟台,264025;烟台师范学院生命科学学院,山东,烟台,264025;烟台师范学院生命科学学院,山东,烟台,264025
摘    要:以平菇PL-39为出发菌株,采用紫外线连续诱变、分离提纯、驯化等方法,以斜面菌丝生长速度和液体摇瓶菌丝生物量及发酵多糖总量为测定目标,筛选出了一株遗传上相对稳定的适应液体培养的平菇菌株———PL-3903。该菌株在综合PDA培养基上生长快,长势旺,菌丝体浓密纯白,经驯化后在液体培养基中发酵生活力强,生物量达2.49 g/100 ml,发酵多糖总量达3.38 g/100 ml。

关 键 词:液体发酵  平菇  诱变选育  生物量
文章编号:0517-6611(2006)09-1858-02
收稿时间:2005-12-28
修稿时间:2005-12-28

Selection of Induced Strain for Liquid Fermentation of Plurotus ostreatus
WANG Shu-fang et al.Selection of Induced Strain for Liquid Fermentation of Plurotus ostreatus[J].Journal of Anhui Agricultural Sciences,2006,34(9):1858-1859.
Authors:WANG Shu-fang
Institution:Life Science College, Yantai Normal University, Yantai, Shandong 264025
Abstract:In this experiment,Pleurotus ostreatus strain PL-39 was used as original strain and a new strain PL-3903 suitable for liquid culture was selected by means of UV continuously induced mutation,isolation,purification and dome stication,and through the measure of growth rate of slanthyhae and polysaccharides quantity of fermentation.The result showed that PL-3903 had a relatively genetic stability and can grow fast and well in the medium with enriched PDA.Its mycelia were dense and white,and its vitality in liquid medium was strong.The biomass was up to 2.49?g/100?ml and total polysaccharides quantity was up to 3.38?g/100?ml.
Keywords:Liquid fermentation  Pleurotus ostreatus  Selection of induced mutation  Biomass
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